Virulence determinants in genetically heterogeneous populations of Aeromonads recovered from an urban lagoon

The diversity and distribution of Aeromonas spp. associated with virulence profiles from the Rodrigo de Freitas Lagoon were investigated using phylogenetic analysis of gyrB/rpoB gene sequences for speciation. The concatenated gyrB/rpoB gene sequences clustered into five species: Aeromonas punctata/c...

Full description

Saved in:
Bibliographic Details
Published in:Journal of water and health 2019-06, Vol.17 (3), p.380-392
Main Authors: de Melo Rodrigues Sobral, Mariana, Barreto, Camila, Bianco, Kayo, de Oliveira, Samara Sant'Anna, Clementino, Maysa Mandetta
Format: Article
Language:English
Subjects:
Aeromonads
Aeromonas
Aeromonas - physiology
Antimicrobial agents
Biofilms
Drinking water
Drug resistance
Epidemiology
Gene sequencing
Genes
Gram-Negative Bacterial Infections
Heterogeneity
Humans
Infections
Lagoons
Microorganisms
Pathogenicity
Pathogens
Phylogenetics
Phylogeny
Population genetics
Profiles
Proteolysis
Public health
RpoB protein
Speciation
Species
Surveys
Virulence
Virulence Factors
Water Microbiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The diversity and distribution of Aeromonas spp. associated with virulence profiles from the Rodrigo de Freitas Lagoon were investigated using phylogenetic analysis of gyrB/rpoB gene sequences for speciation. The concatenated gyrB/rpoB gene sequences clustered into five species: Aeromonas punctata/caviae (n = 37), A. hydrophila (n = 10), A. dhakensis (n = 16), A. jandaei (n = 1) and A. enteropelogenes/trota (n = 3). The virulence genes (atc/aerA/hlyA/asp/amp) resulted in 19 virulence profiles, distributed heterogeneously among the five Aeromonas species. Out of the 67 isolates, 16% presented five distinct profiles carrying four virulence genes and 7% showed all genes investigated. The hemolytic genes were detected as follows: act 54% (37/67), aerA 36% (24/67), hlyA 26% (18/67) and proteolytic genes such as asp 36% (24/57) and amp in 85% (57/67) were widely distributed in lagoon sampling stations. Meanwhile, 88% (59/67) and 92% (62/67) of the isolates showed hemolytic and proteolytic activity, respectively. Our results demonstrated that concatenated sequences of the gyrB and rpoB genes showed to be an adequate approach for the Aeromonas speciation and prevalence. The high heterogeneity of virulence genes among the species resulted in several virulence profiles, as well as high percentages of hemolytic and proteolytic activity, demonstrating the necessity of further epidemiological surveys of Aeromonas species pathogenicity in an aquatic recreational lagoon.
ISSN:1477-8920
1996-7829
DOI:10.2166/wh.2019.288