Bioprospecting microalgae from natural algal bloom for sustainable biomass and biodiesel production

• Published in: Applied microbiology and biotechnology 2019-07, Vol.103 (13), p.5447-5458
• Main Authors: Pandey, Manish K., Dasgupta, Chitralekha Nag, Mishra, Shashank, Srivastava, Manish, Gupta, Vijai Kumar, Suseela, M. R., Ramteke, P. W.
• Format: Article
• Language: English
• Subjects: Algae; Algal blooms; Aquatic microorganisms; Biodiesel fuels; Bioenergy and Biofuels; Biofuels; Biomass; Biomedical and Life Sciences; Bioprospecting; Biotechnology; Diesel; Drinking water; Eutrophication; Fresh water; Freshwater environments; International standards; Life Sciences; Lipids; Microbial Genetics and Genomics; Microbiology; Nitrogen; Phosphorus; Physiological aspects; Production processes; Quality assessment; Urea; Water bloom
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-019-09856-2

Natural algal bloom consists of promising algal species which could be a feasible option for the source of bulk biomass and biodiesel production. It has been found in five natural fresh water algal blooms (Uttar Pradesh, India), containing high nitrogen (N) (4.6 ± 0.32 mg/L) and phosphorus (P) (4.12 ± 0.29 mg/L) concentration during spring (23.9–25.9 °C) and summer season (32.0–35.0 °C). Among the isolated algae from naturally occurring bloom, Chlorella sorokiniana MKP01 exhibited highest biomass (1.02 ± 0.02 g/L) and lipid content (174.1 ± 9.6 mg/L) in untreated tap water and urea/single super phosphate (SSP) in the ratio (2:1). The biodiesel quality was assessed and found to be with the Indian and international standards. Algal bloom was artificially developed in the open pond containing 10,000 l tap water supplemented with Urea/SSP (2:1) for a consistent supply of bulk biomass, yielded 8 kg of total biomass and lipid 1.3 kg.