Loading…
Effect of 4-amino-3-nitrobenzoic acid on the expression level of the trans-sialidase gene in Trypanosoma cruzi epimastigotes
Trans-sialidase of Trypanosoma cruzi (TcTS) is a key enzyme in the infection process from parasite to host; therefore, it has been considered an important target for developing new anti-Chagas drugs. Different compounds with trypanocidal activity and/or inhibition of TcTS have been reported; however...
Saved in:
Published in: | Pakistan journal of pharmaceutical sciences 2019-03, Vol.32 (2 (Supplementary)), p.825-829 |
---|---|
Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Online Access: | Get full text |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
cited_by | |
---|---|
cites | |
container_end_page | 829 |
container_issue | 2 (Supplementary) |
container_start_page | 825 |
container_title | Pakistan journal of pharmaceutical sciences |
container_volume | 32 |
creator | Vázquez-Jiménez, Lenci K Paz-González, Alma D Kashif, Muhammad Juárez-Rendón, Karina J Nogueda-Torres, Benjamín Bocanegra-García, Virgilio Rivera, Gildardo |
description | Trans-sialidase of Trypanosoma cruzi (TcTS) is a key enzyme in the infection process from parasite to host; therefore, it has been considered an important target for developing new anti-Chagas drugs. Different compounds with trypanocidal activity and/or inhibition of TcTS have been reported; however, some benzoic acid derivatives have shown high enzymatic inhibition but low trypanocidal activity and viceversa. These results show that each compound may possess a different mechanism of action. Based on the above, the compound 4-amino-3-nitrobenzoic acid (16), a potent TcTS inhibitor (77% inhibition in enzymatic assays) was selected to evaluate its effects on the expression level of the TS gene in T. cruzi epimastigotes and determine its involvement in the mechanism of action. Results showed an increase in the expression level of the TcTS gene, which confirmed that compound 16, has a direct effect on TcTS. |
format | article |
fullrecord | <record><control><sourceid>gale_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_2232057058</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A587566521</galeid><sourcerecordid>A587566521</sourcerecordid><originalsourceid>FETCH-LOGICAL-g250t-8c579fd2c074b53819ac56971962620a9e31dd32ebfa3816576cc7065cc80ea73</originalsourceid><addsrcrecordid>eNo1UFtLwzAU7oPi5vQvSB59qeSyJM3jGPMCA18m-FbS9KRG2qQ2rbjhjzdjk_NwON8NvnORzQkmJBeYvM-y6xg_MRZLpdRVNmOEYKZkMc9-N9aCGVGwaJnrzvmQs9y7cQgV-ENwBmnjahQ8Gj8AwU8_QIwunS18Q3u0HfFx0D7m0enW1ToCasADch7thn2vfYih08gM08Eh6F2n4-iaMEK8yS6tbiPcnvcie3vc7NbP-fb16WW92uYN5XjMC8OlsjU1WC4rzgqitOFCSaIEFRRrBYzUNaNQWZ1YwaUwRmLBjSkwaMkW2f0ptx_C1wRxLDsXDbSt9hCmWFLKKOYS8yJJH07SRrdQOm9D6mbS1NA5EzxYl_AVLyQXglOSDHfn7KnqoC77IRUc9uX_i9kf1153jA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2232057058</pqid></control><display><type>article</type><title>Effect of 4-amino-3-nitrobenzoic acid on the expression level of the trans-sialidase gene in Trypanosoma cruzi epimastigotes</title><source>EZB Electronic Journals Library</source><creator>Vázquez-Jiménez, Lenci K ; Paz-González, Alma D ; Kashif, Muhammad ; Juárez-Rendón, Karina J ; Nogueda-Torres, Benjamín ; Bocanegra-García, Virgilio ; Rivera, Gildardo</creator><creatorcontrib>Vázquez-Jiménez, Lenci K ; Paz-González, Alma D ; Kashif, Muhammad ; Juárez-Rendón, Karina J ; Nogueda-Torres, Benjamín ; Bocanegra-García, Virgilio ; Rivera, Gildardo</creatorcontrib><description>Trans-sialidase of Trypanosoma cruzi (TcTS) is a key enzyme in the infection process from parasite to host; therefore, it has been considered an important target for developing new anti-Chagas drugs. Different compounds with trypanocidal activity and/or inhibition of TcTS have been reported; however, some benzoic acid derivatives have shown high enzymatic inhibition but low trypanocidal activity and viceversa. These results show that each compound may possess a different mechanism of action. Based on the above, the compound 4-amino-3-nitrobenzoic acid (16), a potent TcTS inhibitor (77% inhibition in enzymatic assays) was selected to evaluate its effects on the expression level of the TS gene in T. cruzi epimastigotes and determine its involvement in the mechanism of action. Results showed an increase in the expression level of the TcTS gene, which confirmed that compound 16, has a direct effect on TcTS.</description><identifier>ISSN: 1011-601X</identifier><identifier>PMID: 31103978</identifier><language>eng</language><publisher>Pakistan: Pakistan Journal of Pharmaceutical Sciences</publisher><subject>Anopheles ; Benzoic acid ; Care and treatment ; Chagas disease ; Enzymes ; Esophagus ; Genetic aspects ; Infection ; Medical research ; Organic acids ; Risk factors ; Trypanosoma cruzi</subject><ispartof>Pakistan journal of pharmaceutical sciences, 2019-03, Vol.32 (2 (Supplementary)), p.825-829</ispartof><rights>COPYRIGHT 2019 Pakistan Journal of Pharmaceutical Sciences</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31103978$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vázquez-Jiménez, Lenci K</creatorcontrib><creatorcontrib>Paz-González, Alma D</creatorcontrib><creatorcontrib>Kashif, Muhammad</creatorcontrib><creatorcontrib>Juárez-Rendón, Karina J</creatorcontrib><creatorcontrib>Nogueda-Torres, Benjamín</creatorcontrib><creatorcontrib>Bocanegra-García, Virgilio</creatorcontrib><creatorcontrib>Rivera, Gildardo</creatorcontrib><title>Effect of 4-amino-3-nitrobenzoic acid on the expression level of the trans-sialidase gene in Trypanosoma cruzi epimastigotes</title><title>Pakistan journal of pharmaceutical sciences</title><addtitle>Pak J Pharm Sci</addtitle><description>Trans-sialidase of Trypanosoma cruzi (TcTS) is a key enzyme in the infection process from parasite to host; therefore, it has been considered an important target for developing new anti-Chagas drugs. Different compounds with trypanocidal activity and/or inhibition of TcTS have been reported; however, some benzoic acid derivatives have shown high enzymatic inhibition but low trypanocidal activity and viceversa. These results show that each compound may possess a different mechanism of action. Based on the above, the compound 4-amino-3-nitrobenzoic acid (16), a potent TcTS inhibitor (77% inhibition in enzymatic assays) was selected to evaluate its effects on the expression level of the TS gene in T. cruzi epimastigotes and determine its involvement in the mechanism of action. Results showed an increase in the expression level of the TcTS gene, which confirmed that compound 16, has a direct effect on TcTS.</description><subject>Anopheles</subject><subject>Benzoic acid</subject><subject>Care and treatment</subject><subject>Chagas disease</subject><subject>Enzymes</subject><subject>Esophagus</subject><subject>Genetic aspects</subject><subject>Infection</subject><subject>Medical research</subject><subject>Organic acids</subject><subject>Risk factors</subject><subject>Trypanosoma cruzi</subject><issn>1011-601X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNo1UFtLwzAU7oPi5vQvSB59qeSyJM3jGPMCA18m-FbS9KRG2qQ2rbjhjzdjk_NwON8NvnORzQkmJBeYvM-y6xg_MRZLpdRVNmOEYKZkMc9-N9aCGVGwaJnrzvmQs9y7cQgV-ENwBmnjahQ8Gj8AwU8_QIwunS18Q3u0HfFx0D7m0enW1ToCasADch7thn2vfYih08gM08Eh6F2n4-iaMEK8yS6tbiPcnvcie3vc7NbP-fb16WW92uYN5XjMC8OlsjU1WC4rzgqitOFCSaIEFRRrBYzUNaNQWZ1YwaUwRmLBjSkwaMkW2f0ptx_C1wRxLDsXDbSt9hCmWFLKKOYS8yJJH07SRrdQOm9D6mbS1NA5EzxYl_AVLyQXglOSDHfn7KnqoC77IRUc9uX_i9kf1153jA</recordid><startdate>20190301</startdate><enddate>20190301</enddate><creator>Vázquez-Jiménez, Lenci K</creator><creator>Paz-González, Alma D</creator><creator>Kashif, Muhammad</creator><creator>Juárez-Rendón, Karina J</creator><creator>Nogueda-Torres, Benjamín</creator><creator>Bocanegra-García, Virgilio</creator><creator>Rivera, Gildardo</creator><general>Pakistan Journal of Pharmaceutical Sciences</general><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20190301</creationdate><title>Effect of 4-amino-3-nitrobenzoic acid on the expression level of the trans-sialidase gene in Trypanosoma cruzi epimastigotes</title><author>Vázquez-Jiménez, Lenci K ; Paz-González, Alma D ; Kashif, Muhammad ; Juárez-Rendón, Karina J ; Nogueda-Torres, Benjamín ; Bocanegra-García, Virgilio ; Rivera, Gildardo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-g250t-8c579fd2c074b53819ac56971962620a9e31dd32ebfa3816576cc7065cc80ea73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Anopheles</topic><topic>Benzoic acid</topic><topic>Care and treatment</topic><topic>Chagas disease</topic><topic>Enzymes</topic><topic>Esophagus</topic><topic>Genetic aspects</topic><topic>Infection</topic><topic>Medical research</topic><topic>Organic acids</topic><topic>Risk factors</topic><topic>Trypanosoma cruzi</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vázquez-Jiménez, Lenci K</creatorcontrib><creatorcontrib>Paz-González, Alma D</creatorcontrib><creatorcontrib>Kashif, Muhammad</creatorcontrib><creatorcontrib>Juárez-Rendón, Karina J</creatorcontrib><creatorcontrib>Nogueda-Torres, Benjamín</creatorcontrib><creatorcontrib>Bocanegra-García, Virgilio</creatorcontrib><creatorcontrib>Rivera, Gildardo</creatorcontrib><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Pakistan journal of pharmaceutical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vázquez-Jiménez, Lenci K</au><au>Paz-González, Alma D</au><au>Kashif, Muhammad</au><au>Juárez-Rendón, Karina J</au><au>Nogueda-Torres, Benjamín</au><au>Bocanegra-García, Virgilio</au><au>Rivera, Gildardo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of 4-amino-3-nitrobenzoic acid on the expression level of the trans-sialidase gene in Trypanosoma cruzi epimastigotes</atitle><jtitle>Pakistan journal of pharmaceutical sciences</jtitle><addtitle>Pak J Pharm Sci</addtitle><date>2019-03-01</date><risdate>2019</risdate><volume>32</volume><issue>2 (Supplementary)</issue><spage>825</spage><epage>829</epage><pages>825-829</pages><issn>1011-601X</issn><abstract>Trans-sialidase of Trypanosoma cruzi (TcTS) is a key enzyme in the infection process from parasite to host; therefore, it has been considered an important target for developing new anti-Chagas drugs. Different compounds with trypanocidal activity and/or inhibition of TcTS have been reported; however, some benzoic acid derivatives have shown high enzymatic inhibition but low trypanocidal activity and viceversa. These results show that each compound may possess a different mechanism of action. Based on the above, the compound 4-amino-3-nitrobenzoic acid (16), a potent TcTS inhibitor (77% inhibition in enzymatic assays) was selected to evaluate its effects on the expression level of the TS gene in T. cruzi epimastigotes and determine its involvement in the mechanism of action. Results showed an increase in the expression level of the TcTS gene, which confirmed that compound 16, has a direct effect on TcTS.</abstract><cop>Pakistan</cop><pub>Pakistan Journal of Pharmaceutical Sciences</pub><pmid>31103978</pmid><tpages>5</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 1011-601X |
ispartof | Pakistan journal of pharmaceutical sciences, 2019-03, Vol.32 (2 (Supplementary)), p.825-829 |
issn | 1011-601X |
language | eng |
recordid | cdi_proquest_miscellaneous_2232057058 |
source | EZB Electronic Journals Library |
subjects | Anopheles Benzoic acid Care and treatment Chagas disease Enzymes Esophagus Genetic aspects Infection Medical research Organic acids Risk factors Trypanosoma cruzi |
title | Effect of 4-amino-3-nitrobenzoic acid on the expression level of the trans-sialidase gene in Trypanosoma cruzi epimastigotes |
url | http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T12%3A57%3A51IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%204-amino-3-nitrobenzoic%20acid%20on%20the%20expression%20level%20of%20the%20trans-sialidase%20gene%20in%20Trypanosoma%20cruzi%20epimastigotes&rft.jtitle=Pakistan%20journal%20of%20pharmaceutical%20sciences&rft.au=V%C3%A1zquez-Jim%C3%A9nez,%20Lenci%20K&rft.date=2019-03-01&rft.volume=32&rft.issue=2%20(Supplementary)&rft.spage=825&rft.epage=829&rft.pages=825-829&rft.issn=1011-601X&rft_id=info:doi/&rft_dat=%3Cgale_proqu%3EA587566521%3C/gale_proqu%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-g250t-8c579fd2c074b53819ac56971962620a9e31dd32ebfa3816576cc7065cc80ea73%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2232057058&rft_id=info:pmid/31103978&rft_galeid=A587566521&rfr_iscdi=true |