Advances in screening enzyme inhibitors by capillary electrophoresis

Capillary electrophoresis (CE) has attracted lots of attention due to its simplicity, low sample consumption, low solvent volume, high resolution, and high speed. Based on these advantages, it has been widely used in enzyme inhibitor screening. There are two main operation modes on enzyme inhibitor...

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Bibliographic Details
Published in:Electrophoresis 2019-08, Vol.40 (16-17), p.2075-2083
Main Authors: Wang, Wei‐Feng, Yang, Jun‐Li
Format: Article
Language:English
Subjects:
Capillary electrophoresis
Electrophoresis
Electrophoresis, Capillary
Enzyme activity
Enzyme Assays
Enzyme inhibitor
Enzyme Inhibitors - analysis
Enzyme Inhibitors - chemistry
Enzyme Inhibitors - metabolism
Enzymes
Enzymes, Immobilized - chemistry
Enzymes, Immobilized - metabolism
Immobilization
Inhibitors
Screening
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Summary:Capillary electrophoresis (CE) has attracted lots of attention due to its simplicity, low sample consumption, low solvent volume, high resolution, and high speed. Based on these advantages, it has been widely used in enzyme inhibitor screening. There are two main operation modes on enzyme inhibitor screening: off‐line (precapillary enzyme assays) in which process CE was used as an analytical tool; online (in‐capillary enzyme assays) which combined the sample injection, mix, reaction, separation, and detection within a single run. Additionally, diverse of new materials were introduced to immobilize enzyme, which has been coupled with CE for the study of enzyme activity and its inhibitor screening. This review gives an overview of the developments and applications for the CE‐based enzyme inhibitor screening.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.201900013