mRNA levels are buffered upon knockdown of RNA decay and translation factors via adjustment of transcription rates in human HepG2 cells

Evidence from yeast and mammals argues the existence of cross-talk between transcription and mRNA decay. Stabilization of transcripts upon depletion of mRNA decay factors generally leads to no changes in mRNA abundance, attributing this to decreased transcription rates. We show that knockdown of hum...

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Bibliographic Details
Published in:RNA biology 2019-09, Vol.16 (9), p.1147-1155
Main Authors: Singh, Pavneet, James, Rob S., Mee, Christopher J, Morozov, Igor Y.
Format: Article
Language:English
Subjects:
Brief Communication
cancer
coupling
Exoribonucleases - genetics
Gene expression
Gene Expression Regulation, Neoplastic - genetics
Hep G2 Cells
human cells
Humans
Microtubule-Associated Proteins - genetics
mRNA decay
Neoplasms - genetics
Peptide Termination Factors - genetics
regulation
RNA Stability - genetics
RNA, Messenger - genetics
transcription
translation
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Summary:Evidence from yeast and mammals argues the existence of cross-talk between transcription and mRNA decay. Stabilization of transcripts upon depletion of mRNA decay factors generally leads to no changes in mRNA abundance, attributing this to decreased transcription rates. We show that knockdown of human XRN1, CNOT6 and ETF1 genes in HepG2 cells led to significant alteration in stability of specific mRNAs, alterations in half-life were inversely associated with transcription rates, mostly not resulting in changes in abundance. We demonstrate the existence of the gene expression buffering mechanism in human cells that responds to both transcript stabilization and destabilization to maintain mRNA abundance via altered transcription rates and may involve translation. We propose that this buffering may hold novel cancer therapeutic targets.
ISSN:1547-6286
1555-8584
DOI:10.1080/15476286.2019.1621121