Baseline separation of α and β‐acids homologues and isomers in hop (Humulus lupulus L.) by CD‐MEKC‐UV

An alternative method for simultaneous baseline separation of α and β‐acids homologues and isomers in hop by CD‐MEKC with UV detection was proposed. The optimized background electrolyte was composed of 30 mmol/L sodium tetraborate solution, 45 mmol/L sodium dodecyl sulfate, 20 mmol/L β‐cyclodextrin...

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Bibliographic Details
Published in:Electrophoresis 2019-07, Vol.40 (14), p.1779-1786
Main Authors: Duarte, Lucas M., Amorim, Tatiane L., Adriano, Luiz H. C., Oliveira, Marcone A. L.
Format: Article
Language:English
Subjects:
Acetonitrile
Acids - analysis
beta-Cyclodextrins - chemistry
Borates - chemistry
CD‐MEKC‐UV
Chromatography, Micellar Electrokinetic Capillary
Cyclodextrins
Design of experiments
Electrophoresis, Capillary
Homology
Hop
Humulones
Humulus - chemistry
Isomerism
Isomers
Lupulones
Separation
Sodium dodecyl sulfate
Sodium Dodecyl Sulfate - chemistry
Sodium tetraborate
Spectrophotometry, Ultraviolet
α and β‐acids
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Summary:An alternative method for simultaneous baseline separation of α and β‐acids homologues and isomers in hop by CD‐MEKC with UV detection was proposed. The optimized background electrolyte was composed of 30 mmol/L sodium tetraborate solution, 45 mmol/L sodium dodecyl sulfate, 20 mmol/L β‐cyclodextrin and 10% v/v acetonitrile. The instrumental conditions were evaluated by using a 33 Box‐Benhken experimental design. In order to demonstrate the applicability of the method, 21 hop samples from different varieties were analyzed. The repeatability intra‐ and interday tests were performed and relative standard deviations lower than 7% for area and migration times were observed. The present method comprehended 8 min analysis time and revealed to be faster and more efficient when compared to previous reports from literature.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.201900101