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Three-Dimensional Protein Cage Array Capable of Active Enzyme Capture and Artificial Chaperone Activity

Development of protein cages for encapsulation of active enzyme cargoes and their subsequent arrangement into a controllable three-dimensional array is highly desirable. However, cargo capture is typically challenging because of difficulties in achieving reversible assembly/disassembly of protein ca...

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Bibliographic Details
Published in:Nano letters 2019-06, Vol.19 (6), p.3918-3924
Main Authors: Chakraborti, Soumyananda, Korpi, Antti, Kumar, Mantu, Stępień, Piotr, Kostiainen, Mauri A, Heddle, Jonathan G
Format: Article
Language:English
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Summary:Development of protein cages for encapsulation of active enzyme cargoes and their subsequent arrangement into a controllable three-dimensional array is highly desirable. However, cargo capture is typically challenging because of difficulties in achieving reversible assembly/disassembly of protein cages in mild conditions. Herein we show that by using an unusual ferritin cage protein that undergoes triggerable assembly under mild conditions, we can achieve reversible filling with protein cargoes including an active enzyme. We demonstrate that these filled cages can be arrayed in three-dimensional crystal lattices and have an additional chaperone-like effect, increasing both thermostability and enzymatic activity of the encapsulated enzyme.
ISSN:1530-6984
1530-6992
DOI:10.1021/acs.nanolett.9b01148