Loading…

Three-Dimensional Protein Cage Array Capable of Active Enzyme Capture and Artificial Chaperone Activity

Development of protein cages for encapsulation of active enzyme cargoes and their subsequent arrangement into a controllable three-dimensional array is highly desirable. However, cargo capture is typically challenging because of difficulties in achieving reversible assembly/disassembly of protein ca...

Full description

Saved in:
Bibliographic Details
Published in:Nano letters 2019-06, Vol.19 (6), p.3918-3924
Main Authors: Chakraborti, Soumyananda, Korpi, Antti, Kumar, Mantu, Stępień, Piotr, Kostiainen, Mauri A, Heddle, Jonathan G
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-a460t-6027a67dd7c659127832f0afe99b7b4261c8256bbd68a2baaa31534faaecc3423
cites cdi_FETCH-LOGICAL-a460t-6027a67dd7c659127832f0afe99b7b4261c8256bbd68a2baaa31534faaecc3423
container_end_page 3924
container_issue 6
container_start_page 3918
container_title Nano letters
container_volume 19
creator Chakraborti, Soumyananda
Korpi, Antti
Kumar, Mantu
Stępień, Piotr
Kostiainen, Mauri A
Heddle, Jonathan G
description Development of protein cages for encapsulation of active enzyme cargoes and their subsequent arrangement into a controllable three-dimensional array is highly desirable. However, cargo capture is typically challenging because of difficulties in achieving reversible assembly/disassembly of protein cages in mild conditions. Herein we show that by using an unusual ferritin cage protein that undergoes triggerable assembly under mild conditions, we can achieve reversible filling with protein cargoes including an active enzyme. We demonstrate that these filled cages can be arrayed in three-dimensional crystal lattices and have an additional chaperone-like effect, increasing both thermostability and enzymatic activity of the encapsulated enzyme.
doi_str_mv 10.1021/acs.nanolett.9b01148
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_2232109662</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2232109662</sourcerecordid><originalsourceid>FETCH-LOGICAL-a460t-6027a67dd7c659127832f0afe99b7b4261c8256bbd68a2baaa31534faaecc3423</originalsourceid><addsrcrecordid>eNp9kMtOwzAQRS0EoqXwBwhlySbFdhInWValPKRKsCjraOJMWleJXWwHKXw9qfpYsvJIPveO5hByz-iUUc6eQLqpBm0a9H6al5SxOLsgY5ZENBR5zi_PcxaPyI1zW0ppHiX0mowixliaJtmYrFcbixg-qxa1U0ZDE3xa41HpYA5rDGbWQj-MOygbDEwdzKRXPxgs9G_f4v7DdxYD0NWAelUrqYaK-QZ2aI3GA658f0uuamgc3h3fCfl6Wazmb-Hy4_V9PluGEAvqQ0F5CiKtqlSKJGc8zSJeU6gxz8u0jLlgMuOJKMtKZMBLAIiGI-MaAKWMYh5NyOOhd2fNd4fOF61yEpsGNJrOFZxHnNFciD0aH1BpjXMW62JnVQu2Lxgt9oqLQXFxUlwcFQ-xh-OGrmyxOodOTgeAHoB9fGs6O0h1_3f-AQzpjAE</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2232109662</pqid></control><display><type>article</type><title>Three-Dimensional Protein Cage Array Capable of Active Enzyme Capture and Artificial Chaperone Activity</title><source>American Chemical Society:Jisc Collections:American Chemical Society Read &amp; Publish Agreement 2022-2024 (Reading list)</source><creator>Chakraborti, Soumyananda ; Korpi, Antti ; Kumar, Mantu ; Stępień, Piotr ; Kostiainen, Mauri A ; Heddle, Jonathan G</creator><creatorcontrib>Chakraborti, Soumyananda ; Korpi, Antti ; Kumar, Mantu ; Stępień, Piotr ; Kostiainen, Mauri A ; Heddle, Jonathan G</creatorcontrib><description>Development of protein cages for encapsulation of active enzyme cargoes and their subsequent arrangement into a controllable three-dimensional array is highly desirable. However, cargo capture is typically challenging because of difficulties in achieving reversible assembly/disassembly of protein cages in mild conditions. Herein we show that by using an unusual ferritin cage protein that undergoes triggerable assembly under mild conditions, we can achieve reversible filling with protein cargoes including an active enzyme. We demonstrate that these filled cages can be arrayed in three-dimensional crystal lattices and have an additional chaperone-like effect, increasing both thermostability and enzymatic activity of the encapsulated enzyme.</description><identifier>ISSN: 1530-6984</identifier><identifier>EISSN: 1530-6992</identifier><identifier>DOI: 10.1021/acs.nanolett.9b01148</identifier><identifier>PMID: 31117758</identifier><language>eng</language><publisher>United States: American Chemical Society</publisher><subject>Amino Acid Sequence ; Animals ; Archaeal Proteins - chemistry ; Archaeoglobus fulgidus - chemistry ; Bacterial Proteins - chemistry ; Delayed-Action Preparations - chemistry ; Enzyme Stability ; Enzymes, Immobilized - administration &amp; dosage ; Enzymes, Immobilized - chemistry ; Ferritins - chemistry ; Green Fluorescent Proteins - administration &amp; dosage ; Green Fluorescent Proteins - chemistry ; Models, Molecular ; Muramidase - administration &amp; dosage ; Muramidase - chemistry ; Nanostructures - chemistry ; Protein Binding ; Protein Folding ; Thermotoga maritima - chemistry</subject><ispartof>Nano letters, 2019-06, Vol.19 (6), p.3918-3924</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a460t-6027a67dd7c659127832f0afe99b7b4261c8256bbd68a2baaa31534faaecc3423</citedby><cites>FETCH-LOGICAL-a460t-6027a67dd7c659127832f0afe99b7b4261c8256bbd68a2baaa31534faaecc3423</cites><orcidid>0000-0003-0994-9928 ; 0000-0002-8282-2379</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31117758$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Chakraborti, Soumyananda</creatorcontrib><creatorcontrib>Korpi, Antti</creatorcontrib><creatorcontrib>Kumar, Mantu</creatorcontrib><creatorcontrib>Stępień, Piotr</creatorcontrib><creatorcontrib>Kostiainen, Mauri A</creatorcontrib><creatorcontrib>Heddle, Jonathan G</creatorcontrib><title>Three-Dimensional Protein Cage Array Capable of Active Enzyme Capture and Artificial Chaperone Activity</title><title>Nano letters</title><addtitle>Nano Lett</addtitle><description>Development of protein cages for encapsulation of active enzyme cargoes and their subsequent arrangement into a controllable three-dimensional array is highly desirable. However, cargo capture is typically challenging because of difficulties in achieving reversible assembly/disassembly of protein cages in mild conditions. Herein we show that by using an unusual ferritin cage protein that undergoes triggerable assembly under mild conditions, we can achieve reversible filling with protein cargoes including an active enzyme. We demonstrate that these filled cages can be arrayed in three-dimensional crystal lattices and have an additional chaperone-like effect, increasing both thermostability and enzymatic activity of the encapsulated enzyme.</description><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Archaeal Proteins - chemistry</subject><subject>Archaeoglobus fulgidus - chemistry</subject><subject>Bacterial Proteins - chemistry</subject><subject>Delayed-Action Preparations - chemistry</subject><subject>Enzyme Stability</subject><subject>Enzymes, Immobilized - administration &amp; dosage</subject><subject>Enzymes, Immobilized - chemistry</subject><subject>Ferritins - chemistry</subject><subject>Green Fluorescent Proteins - administration &amp; dosage</subject><subject>Green Fluorescent Proteins - chemistry</subject><subject>Models, Molecular</subject><subject>Muramidase - administration &amp; dosage</subject><subject>Muramidase - chemistry</subject><subject>Nanostructures - chemistry</subject><subject>Protein Binding</subject><subject>Protein Folding</subject><subject>Thermotoga maritima - chemistry</subject><issn>1530-6984</issn><issn>1530-6992</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><recordid>eNp9kMtOwzAQRS0EoqXwBwhlySbFdhInWValPKRKsCjraOJMWleJXWwHKXw9qfpYsvJIPveO5hByz-iUUc6eQLqpBm0a9H6al5SxOLsgY5ZENBR5zi_PcxaPyI1zW0ppHiX0mowixliaJtmYrFcbixg-qxa1U0ZDE3xa41HpYA5rDGbWQj-MOygbDEwdzKRXPxgs9G_f4v7DdxYD0NWAelUrqYaK-QZ2aI3GA658f0uuamgc3h3fCfl6Wazmb-Hy4_V9PluGEAvqQ0F5CiKtqlSKJGc8zSJeU6gxz8u0jLlgMuOJKMtKZMBLAIiGI-MaAKWMYh5NyOOhd2fNd4fOF61yEpsGNJrOFZxHnNFciD0aH1BpjXMW62JnVQu2Lxgt9oqLQXFxUlwcFQ-xh-OGrmyxOodOTgeAHoB9fGs6O0h1_3f-AQzpjAE</recordid><startdate>20190612</startdate><enddate>20190612</enddate><creator>Chakraborti, Soumyananda</creator><creator>Korpi, Antti</creator><creator>Kumar, Mantu</creator><creator>Stępień, Piotr</creator><creator>Kostiainen, Mauri A</creator><creator>Heddle, Jonathan G</creator><general>American Chemical Society</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0003-0994-9928</orcidid><orcidid>https://orcid.org/0000-0002-8282-2379</orcidid></search><sort><creationdate>20190612</creationdate><title>Three-Dimensional Protein Cage Array Capable of Active Enzyme Capture and Artificial Chaperone Activity</title><author>Chakraborti, Soumyananda ; Korpi, Antti ; Kumar, Mantu ; Stępień, Piotr ; Kostiainen, Mauri A ; Heddle, Jonathan G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a460t-6027a67dd7c659127832f0afe99b7b4261c8256bbd68a2baaa31534faaecc3423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Archaeal Proteins - chemistry</topic><topic>Archaeoglobus fulgidus - chemistry</topic><topic>Bacterial Proteins - chemistry</topic><topic>Delayed-Action Preparations - chemistry</topic><topic>Enzyme Stability</topic><topic>Enzymes, Immobilized - administration &amp; dosage</topic><topic>Enzymes, Immobilized - chemistry</topic><topic>Ferritins - chemistry</topic><topic>Green Fluorescent Proteins - administration &amp; dosage</topic><topic>Green Fluorescent Proteins - chemistry</topic><topic>Models, Molecular</topic><topic>Muramidase - administration &amp; dosage</topic><topic>Muramidase - chemistry</topic><topic>Nanostructures - chemistry</topic><topic>Protein Binding</topic><topic>Protein Folding</topic><topic>Thermotoga maritima - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chakraborti, Soumyananda</creatorcontrib><creatorcontrib>Korpi, Antti</creatorcontrib><creatorcontrib>Kumar, Mantu</creatorcontrib><creatorcontrib>Stępień, Piotr</creatorcontrib><creatorcontrib>Kostiainen, Mauri A</creatorcontrib><creatorcontrib>Heddle, Jonathan G</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nano letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chakraborti, Soumyananda</au><au>Korpi, Antti</au><au>Kumar, Mantu</au><au>Stępień, Piotr</au><au>Kostiainen, Mauri A</au><au>Heddle, Jonathan G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Three-Dimensional Protein Cage Array Capable of Active Enzyme Capture and Artificial Chaperone Activity</atitle><jtitle>Nano letters</jtitle><addtitle>Nano Lett</addtitle><date>2019-06-12</date><risdate>2019</risdate><volume>19</volume><issue>6</issue><spage>3918</spage><epage>3924</epage><pages>3918-3924</pages><issn>1530-6984</issn><eissn>1530-6992</eissn><abstract>Development of protein cages for encapsulation of active enzyme cargoes and their subsequent arrangement into a controllable three-dimensional array is highly desirable. However, cargo capture is typically challenging because of difficulties in achieving reversible assembly/disassembly of protein cages in mild conditions. Herein we show that by using an unusual ferritin cage protein that undergoes triggerable assembly under mild conditions, we can achieve reversible filling with protein cargoes including an active enzyme. We demonstrate that these filled cages can be arrayed in three-dimensional crystal lattices and have an additional chaperone-like effect, increasing both thermostability and enzymatic activity of the encapsulated enzyme.</abstract><cop>United States</cop><pub>American Chemical Society</pub><pmid>31117758</pmid><doi>10.1021/acs.nanolett.9b01148</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0003-0994-9928</orcidid><orcidid>https://orcid.org/0000-0002-8282-2379</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1530-6984
ispartof Nano letters, 2019-06, Vol.19 (6), p.3918-3924
issn 1530-6984
1530-6992
language eng
recordid cdi_proquest_miscellaneous_2232109662
source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Amino Acid Sequence
Animals
Archaeal Proteins - chemistry
Archaeoglobus fulgidus - chemistry
Bacterial Proteins - chemistry
Delayed-Action Preparations - chemistry
Enzyme Stability
Enzymes, Immobilized - administration & dosage
Enzymes, Immobilized - chemistry
Ferritins - chemistry
Green Fluorescent Proteins - administration & dosage
Green Fluorescent Proteins - chemistry
Models, Molecular
Muramidase - administration & dosage
Muramidase - chemistry
Nanostructures - chemistry
Protein Binding
Protein Folding
Thermotoga maritima - chemistry
title Three-Dimensional Protein Cage Array Capable of Active Enzyme Capture and Artificial Chaperone Activity
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T13%3A30%3A02IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Three-Dimensional%20Protein%20Cage%20Array%20Capable%20of%20Active%20Enzyme%20Capture%20and%20Artificial%20Chaperone%20Activity&rft.jtitle=Nano%20letters&rft.au=Chakraborti,%20Soumyananda&rft.date=2019-06-12&rft.volume=19&rft.issue=6&rft.spage=3918&rft.epage=3924&rft.pages=3918-3924&rft.issn=1530-6984&rft.eissn=1530-6992&rft_id=info:doi/10.1021/acs.nanolett.9b01148&rft_dat=%3Cproquest_cross%3E2232109662%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-a460t-6027a67dd7c659127832f0afe99b7b4261c8256bbd68a2baaa31534faaecc3423%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2232109662&rft_id=info:pmid/31117758&rfr_iscdi=true