Proteome Changes Paralleling the Olfactory Conditioning in the Forager Honey Bee and Provision of a Brain Proteomics Dataset

The olfactory conditioning of the bee proboscis extension reflex (PER) is extensively used as a paradigm in associative learning of invertebrates but with limited molecular investigations. To investigate which protein changes are linked to olfactory conditioning, a non‐sophisticated conditioning mod...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2019-07, Vol.19 (13), p.e1900094-n/a
Main Authors: Sialana, Fernando J., Ribeiroda Silva Menegasso, Anally, Smidak, Roman, Hussein, Ahmed M., Zavadil, Michael, Rattei, Thomas, Lubec, Gert, Sergio Palma, Mario, Lubec, Jana
Format: Article
Language:English
Subjects:
Animals
Apis mellifera
Associative learning
ATP-Binding Cassette Transporters - metabolism
Bees
Bees - physiology
Brain
Brain - physiology
Conditioning
Conditioning, Classical - physiology
Datasets
Fatty acids
Foraging behavior
honeybee brain
Invertebrates
Isotope Labeling
Neurotransmitters
olfactory conditioning
Olfactory discrimination learning
Proboscis
proboscis extension reflex
Proteins
Proteome - metabolism
Proteomics
Qc-SNARE Proteins - metabolism
quantitative proteomics
R-SNARE Proteins - metabolism
Smell - physiology
SNAP receptors
Stable isotopes
Sucrose
Sugar
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Summary:The olfactory conditioning of the bee proboscis extension reflex (PER) is extensively used as a paradigm in associative learning of invertebrates but with limited molecular investigations. To investigate which protein changes are linked to olfactory conditioning, a non‐sophisticated conditioning model is applied using the PER in the honeybee (Apis mellifera). Foraging honeybees are assigned into three groups based on the reflex behavior and training: conditioned using 2‐octanone (PER‐conditioned), and sucrose and water controls. Thereafter, the brain synaptosomal proteins are isolated and analyzed by quantitative proteomics using stable isotope labeling (TMT). Additionally, the complex proteome dataset of the bee brain is generated with a total number of 5411 protein groups, including key players in neurotransmitter signaling. The most significant categories affected during olfactory conditioning are associated with “SNARE interactions in vesicular transport” (BET1 and VAMP7), ABC transporters, and fatty acid degradation pathways.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.201900094