Recovery of Muscovy duck-origin goose parvovirus from an infectious clone containing an E-box motif (CACATG) deletion within the left terminal region

• Published in: Molecular and cellular probes 2019-08, Vol.46, p.101410-101410, Article 101410
• Main Authors: Wang, Shao, Xiao, Shifeng, Cheng, Xiaoxia, Chen, Shaoying, Zhu, Xiaoli, Lin, Fengqiang, Chen, Shilong
• Format: Article
• Language: English
• Subjects: Animals; Cell Cycle Checkpoints - genetics; Communicable Diseases - genetics; Communicable Diseases - virology; Ducks - genetics; Ducks - virology; E-box motif; Embryo, Nonmammalian - virology; Geese - genetics; Geese - virology; Genome, Viral - genetics; Goose parvovirus; Infectious clone; Liver - virology; Mesenchymal Stem Cells - virology; Muscovy duck; Parvovirinae - genetics; Parvovirinae - pathogenicity; Plasmids - genetics; Poultry Diseases - genetics; Poultry Diseases - virology; Resting Phase, Cell Cycle - genetics; S Phase - genetics; Sequence Deletion
• ISSN: 0890-8508; 1096-1194
• DOI: 10.1016/j.mcp.2019.05.006

Muscovy duck-origin goose parvovirus (MDGPV) is a causative agent of MDGPV-associated Derzsy's disease. To evalute the role of the cis-acting element E-box (CACATG) deletion on MDGPV eplication, an infectious plasmid clone p-PTΔE287, having one E-box deletion at nucleotide (nt) 287 of the left inverted terminal repeat sequence (L-ITR), was constructed by overlap extension PCR deleting the 287CACATG292 motif from the plasmid pMDGPVPT containing the full-length genome of the virulent MDGPV strain PT. The p-PTΔE287 plasmid was transfected into 9-day-old non-immune Muscovy duck embryos via the yolk sac, resulting in successful rescue of the deletion mutant virus r-PTΔE287. Compared with its parental virus PT, the virulence and the replication ability of r-PTΔE287 were reduced. In addition, we examined the ability of r-PTΔE287 to manipulate cell cycle progression. The results showed that r-PTΔE287 replication results in G0/G1 phase accumulation of infected duck embryo liver mesenchymal stem cells (BMSCs) and that this accumulation is caused by the prevention of cell cycle entry from G0/G1 phase into S phase. Taken together, introducing 287CACATG292 element deletion into MDGPV PT genomic DNA that induced rescued mutant virus (r-PTΔE287) cell cycle arrest function at the G0/G1 phase, which might inhibit MDGPV replication and virus progeny production. This study laid the foundation for further understanding of the relationship between E-box deletion in the L-ITR and MDGPV virulence. •We constructed an E-box motif (CACATG)-deleted MDGPV.•Deletion of E-box (E287) decreased MDGPV replication in Muscovy duck embryos.•The mutant virus r-PTΔE287 may induce cell cycle arrest at G0/G1 phase.