Expression and clinical significance of SNAI1 and ZEB1 genes in acute myeloid leukemia patients

• Published in: Molecular biology reports 2019-08, Vol.46 (4), p.4625-4630
• Main Authors: Shousha, Wafaa Ghoneim, Ramadan, Shimaa Shawki, EL-Saiid, Abeer Salah, Abdelmoneim, Ahmed Essmat, Abbas, Marwa Ahmed
• Format: Article
• Language: English
• Subjects: Acute myeloid leukemia; Adult; Animal Anatomy; Animal Biochemistry; Antigens, CD - blood; Biomarkers, Tumor - biosynthesis; Biomarkers, Tumor - genetics; Biomedical and Life Sciences; Cadherins - blood; Clinical significance; E-cadherin; Enzyme-linked immunosorbent assay; Female; Histology; Humans; Leukemia; Leukemia, Myeloid, Acute - blood; Leukemia, Myeloid, Acute - genetics; Life Sciences; Male; Methods Paper; Middle Aged; Morphology; Myeloid leukemia; Prognosis; Serum levels; Snail Family Transcription Factors - blood; Snail Family Transcription Factors - genetics; Snail Family Transcription Factors - metabolism; Snail protein; Transcriptome; Zinc Finger E-box-Binding Homeobox 1 - blood; Zinc Finger E-box-Binding Homeobox 1 - genetics; Zinc Finger E-box-Binding Homeobox 1 - metabolism
• ISSN: 0301-4851; 1573-4978
• DOI: 10.1007/s11033-019-04839-y

Acute myeloid leukemia (AML) is the most common type of acute leukemia in adults, it represents nearly 32% of all new cases of leukemia. This study aimed to evaluate the SNAI1 and ZEB1 genes expression in AML patients and determine their diagnostic and prognostic significance. We determined the expression of SNAI1 and ZEB1 genes and serum E-cadherin levels in early diagnosed patients with AML. Sixty early diagnosed AML patients and 20 healthy subjects were enrolled in this study, SNAI1 and ZEB1 genes expression was determined by Real-time PCR while E-Cadherin serum levels were determined by ELISA. The results of this study demonstrated that, all AML patients positively expressed the SNAI1 gene with fold change 2.6. While, the ZEB1 expression was positive in 56.7% of the patients with fold change 1.8. SNAI1 and ZEB1 genes were highly expressed in M5 subtype (FC = 13.8 and 9.3, respectively). On the other hand, serum E-cadherin concentrations of the AML patients showed decrease when compared with those of the control but the decrease was not reach to the significance level. The findings of this study suggest inclusion of SNAI1 and ZEB1 genes expression in the cluster of potential genetic biomarkers to be studied in AML cases as diagnostic and prognostic markers.