Proanthocyanidin-chitosan composite nanoparticles prevent bacterial invasion and colonization of gut epithelial cells by extra-intestinal pathogenic Escherichia coli

Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs) were formulated using 2:1, 5:1, 10:1, 15:1 20:1, 25:1, and 30:1 PAC to CHT weight ratio to form round shaped particles. The PAC-CHT NPs were characterized by size, polydispersity, surface charge, morphology, and PAC content. P...

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Bibliographic Details
Published in:International journal of biological macromolecules 2019-08, Vol.135, p.630-636
Main Authors: Alfaro-Viquez, Emilia, Esquivel-Alvarado, Daniel, Madrigal-Carballo, Sergio, Krueger, Christian G., Reed, Jess D.
Format: Article
Language:English
Subjects:
Agglutination
Chitosan
Chitosan - chemistry
Epithelial Cells - microbiology
Escherichia coli - drug effects
Escherichia coli - physiology
Extra-intestinal pathogenic Escherichia coli
Intestines - cytology
Intestines - microbiology
Invasion
Nanoparticles - chemistry
Proanthocyanidins
Proanthocyanidins - chemistry
Proanthocyanidins - pharmacology
Vaccinium macrocarpon - chemistry
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Summary:Cranberry proanthocyanidin-chitosan composite nanoparticles (PAC-CHT NPs) were formulated using 2:1, 5:1, 10:1, 15:1 20:1, 25:1, and 30:1 PAC to CHT weight ratio to form round shaped particles. The PAC-CHT NPs were characterized by size, polydispersity, surface charge, morphology, and PAC content. PAC-CHT NPs bioactivity was measured by agglutination of extra-intestinal pathogenic Escherichia coli (ExPEC) and inhibition of gut epithelial cell invasion by ExPEC. Results indicate that by increasing the PAC to CHT ratio 10:1 to 30:1 formed stable nanoparticles with diameters of 122.8 to 618.7 nm, a polydispersity index of approximated 0.4 to 0.5, and a zeta potential of 34.5 to 54.4 mV. PAC-CHT NPs ratio 30:1 agglutinated ExPEC and decreased the ability of ExPEC to invade epithelial cells in a dose-dependent manner. PAC-CHT NPs ratio 10:1 to 30:1 form stable, round-shaped, and bioactive nanoparticles for potential applications in the treatment of ExPEC bacterial infections. [Display omitted]
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2019.04.170