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Green synthesis of silver nanoparticles: effect of synthesis reaction parameters on antimicrobial activity
In this work, the biosynthesis of silver nanoparticles by Galega officinalis extract using AgNO 3 as a precursor was reported. The reaction parameters for the biosynthesis and efficiency in their antimicrobial control against Escherichia coli , Staphylococcus aureus and Pseudomonas syringae were de...
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Published in: | World journal of microbiology & biotechnology 2019-06, Vol.35 (6), p.88-9, Article 88 |
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Main Authors: | , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | In this work, the biosynthesis of silver nanoparticles by
Galega officinalis
extract using AgNO
3
as a precursor was reported. The reaction parameters for the biosynthesis and efficiency in their antimicrobial control against
Escherichia coli
,
Staphylococcus aureus
and
Pseudomonas syringae
were determined. For biosynthesis, a central composite design combined with response surface methodology was used to optimize the process parameters (pH, AgNO
3
and extract concentration), and the design was assessed through the size distribution, zeta potential and polydispersity index of the nanoparticles. The results demonstrated that at pH 11, 1.6 mM of AgNO
3
and 15% vv
−1
of
G. officinalis
extract were the optimal reaction parameters. Transmission electron microscope (TEM) images and X-ray diffraction (XRD) confirmed the formation of small spherical silver nanoparticles. Antimicrobial assays showed a high inhibitory effect against
E. coli
,
S. aureus
and
P. syringae
, and that effect was larger with silver nanoparticles of a smaller size (23 nm). This work demonstrates that
G. officinalis
extract is a feasible medium for the synthesis of silver nanoparticles and that the control of the reaction parameters can determine the nanoparticle characteristics and therefore their antimicrobial effectiveness.
Graphical abstract |
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ISSN: | 0959-3993 1573-0972 |
DOI: | 10.1007/s11274-019-2664-3 |