Loading…

Green synthesis of silver nanoparticles: effect of synthesis reaction parameters on antimicrobial activity

In this work, the biosynthesis of silver nanoparticles by Galega officinalis extract using AgNO 3  as a precursor was reported. The reaction parameters for the biosynthesis and efficiency in their antimicrobial control against Escherichia coli , Staphylococcus aureus and Pseudomonas syringae were de...

Full description

Saved in:
Bibliographic Details
Published in:World journal of microbiology & biotechnology 2019-06, Vol.35 (6), p.88-9, Article 88
Main Authors: Manosalva, Nixson, Tortella, Gonzalo, Cristina Diez, María, Schalchli, Heidi, Seabra, Amedea B., Durán, Nelson, Rubilar, Olga
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this work, the biosynthesis of silver nanoparticles by Galega officinalis extract using AgNO 3  as a precursor was reported. The reaction parameters for the biosynthesis and efficiency in their antimicrobial control against Escherichia coli , Staphylococcus aureus and Pseudomonas syringae were determined. For biosynthesis, a central composite design combined with response surface methodology was used to optimize the process parameters (pH, AgNO 3 and extract concentration), and the design was assessed through the size distribution, zeta potential and polydispersity index of the nanoparticles. The results demonstrated that at pH 11, 1.6 mM of AgNO 3 and 15% vv −1 of G. officinalis extract were the optimal reaction parameters. Transmission electron microscope (TEM) images and X-ray diffraction (XRD) confirmed the formation of small spherical silver nanoparticles. Antimicrobial assays showed a high inhibitory effect against E. coli , S. aureus and P. syringae , and that effect was larger with silver nanoparticles of a smaller size (23 nm). This work demonstrates that G. officinalis extract is a feasible medium for the synthesis of silver nanoparticles and that the control of the reaction parameters can determine the nanoparticle characteristics and therefore their antimicrobial effectiveness. Graphical abstract
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-019-2664-3