Green synthesis of silver nanoparticles: effect of synthesis reaction parameters on antimicrobial activity

In this work, the biosynthesis of silver nanoparticles by Galega officinalis extract using AgNO 3  as a precursor was reported. The reaction parameters for the biosynthesis and efficiency in their antimicrobial control against Escherichia coli , Staphylococcus aureus and Pseudomonas syringae were de...

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Published in:World journal of microbiology & biotechnology 2019-06, Vol.35 (6), p.88-9, Article 88
Main Authors: Manosalva, Nixson, Tortella, Gonzalo, Cristina Diez, María, Schalchli, Heidi, Seabra, Amedea B., Durán, Nelson, Rubilar, Olga
Format: Article
Language:English
Subjects:
Antiinfectives and antibacterials
Antimicrobial activity
Antimicrobial agents
Applied Microbiology
Biochemistry
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Chemical synthesis
E coli
Environmental Engineering/Biotechnology
Escherichia coli
Image transmission
Life Sciences
Microbiology
Nanoparticles
Optimization
Original Paper
Pathogens
pH effects
Polydispersity
Process parameters
Response surface methodology
Silver
Size distribution
X-ray diffraction
Zeta potential
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container_title World journal of microbiology & biotechnology
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Tortella, Gonzalo
Cristina Diez, María
Schalchli, Heidi
Seabra, Amedea B.
Durán, Nelson
Rubilar, Olga
description In this work, the biosynthesis of silver nanoparticles by Galega officinalis extract using AgNO 3  as a precursor was reported. The reaction parameters for the biosynthesis and efficiency in their antimicrobial control against Escherichia coli , Staphylococcus aureus and Pseudomonas syringae were determined. For biosynthesis, a central composite design combined with response surface methodology was used to optimize the process parameters (pH, AgNO 3 and extract concentration), and the design was assessed through the size distribution, zeta potential and polydispersity index of the nanoparticles. The results demonstrated that at pH 11, 1.6 mM of AgNO 3 and 15% vv −1 of G. officinalis extract were the optimal reaction parameters. Transmission electron microscope (TEM) images and X-ray diffraction (XRD) confirmed the formation of small spherical silver nanoparticles. Antimicrobial assays showed a high inhibitory effect against E. coli , S. aureus and P. syringae , and that effect was larger with silver nanoparticles of a smaller size (23 nm). This work demonstrates that G. officinalis extract is a feasible medium for the synthesis of silver nanoparticles and that the control of the reaction parameters can determine the nanoparticle characteristics and therefore their antimicrobial effectiveness. Graphical abstract
doi_str_mv 10.1007/s11274-019-2664-3
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The reaction parameters for the biosynthesis and efficiency in their antimicrobial control against Escherichia coli , Staphylococcus aureus and Pseudomonas syringae were determined. For biosynthesis, a central composite design combined with response surface methodology was used to optimize the process parameters (pH, AgNO 3 and extract concentration), and the design was assessed through the size distribution, zeta potential and polydispersity index of the nanoparticles. The results demonstrated that at pH 11, 1.6 mM of AgNO 3 and 15% vv −1 of G. officinalis extract were the optimal reaction parameters. Transmission electron microscope (TEM) images and X-ray diffraction (XRD) confirmed the formation of small spherical silver nanoparticles. Antimicrobial assays showed a high inhibitory effect against E. coli , S. aureus and P. syringae , and that effect was larger with silver nanoparticles of a smaller size (23 nm). 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subjects Antiinfectives and antibacterials
Antimicrobial activity
Antimicrobial agents
Applied Microbiology
Biochemistry
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Chemical synthesis
E coli
Environmental Engineering/Biotechnology
Escherichia coli
Image transmission
Life Sciences
Microbiology
Nanoparticles
Optimization
Original Paper
Pathogens
pH effects
Polydispersity
Process parameters
Response surface methodology
Silver
Size distribution
X-ray diffraction
Zeta potential
title Green synthesis of silver nanoparticles: effect of synthesis reaction parameters on antimicrobial activity
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