Protective effect of gallic acid on apoptosis of sperm and in vitro fertilization in adult male mice treated with cyclophosphamide

Background Alteration of free radicals (reactive oxygen species) causes mammals' sperm damage. Gallic acid (GA) is known as an antioxidant which is effective against oxidative stress. The purpose of this study was to evaluate the antioxidant effects of GA on the sperm apoptosis and in vitro fer...

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Published in:Journal of cellular biochemistry 2019-10, Vol.120 (10), p.17250-17257
Main Authors: Mehraban, Zahra, Ghaffari Novin, Marefat, Golmohammadi, Mohammad Ghasem, Sagha, Mohsen, Pouriran, Khashayar, Nazarian, Hamid
Format: Article
Language:English
Subjects:
Antioxidants
Apoptosis
Cyclophosphamide
Fertility
Flow cytometry
Free radicals
Gallic acid
In vitro fertilization
IVF
Medical research
Mice
Oxidative stress
Parameters
Reactive oxygen species
Sperm
spermatozoa
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Summary:Background Alteration of free radicals (reactive oxygen species) causes mammals' sperm damage. Gallic acid (GA) is known as an antioxidant which is effective against oxidative stress. The purpose of this study was to evaluate the antioxidant effects of GA on the sperm apoptosis and in vitro fertilization (IVF) in adult male mice treated with cyclophosphamide (CP). Materials and Methods Following a pilot study to find the dose responses of GA, 40 adult male naval medical research institute (NMRI) mice (32 ± 3 g) were divided into five groups (n = 8): control, sham (normal saline, NS: 0.2 mL per day), CP (15 mg kg–1 per week; intraperitoneal, IP), GA (12.5 mg kg –1 per day; IP), and GA+CP. After the treatment, sperm parameters were analyzed. The apoptosis of sperm was measured by Annexin‐PI staining method followed by flow cytometry detection. Fertility was assessed by IVF method among the groups. Results The difference in sperm parameter and fertility rate between the control (% 80.05 ± 6.53) and cyclophosphomide groups (% 51.82 ± 10.78) was significant (P 
ISSN:0730-2312
1097-4644
DOI:10.1002/jcb.28987