Factors affecting aflatoxin B1 removal by flavobacterium aurantiacum

This study was conducted to investigate several factors affecting the removal of aflatoxin B1 by Flavobacterium aurantiacum NRRL B-184. A simple spectrophotometric procedure was evaluated and compared to an established high-performance liquid chromatography (HPLC) method and found to be useful for d...

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Bibliographic Details
Published in:Journal of food protection 1995-01, Vol.58 (1), p.91-94
Main Authors: Line, J.E. (University of Georgia Experiment Station, Griffin, GA.), Brackett, R.E
Format: Article
Language:English
Subjects:
AFLATOXINAS
AFLATOXINE
ALIMENTOS
Biological and medical sciences
CHROMATOGRAPHIE LIQUIDE HAUTE PRESS
CROMATOGRAFIA LIQUIDA ALTA PRESION
DESINTOXICACION
DETOXIFICATION
FLAVOBACTERIUM
Food industries
Food toxicology
Fundamental and applied biological sciences. Psychology
MICROBIOLOGIA
MICROBIOLOGIE
PRODUIT ALIMENTAIRE
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Summary:This study was conducted to investigate several factors affecting the removal of aflatoxin B1 by Flavobacterium aurantiacum NRRL B-184. A simple spectrophotometric procedure was evaluated and compared to an established high-performance liquid chromatography (HPLC) method and found to be useful for determining aflatoxin concentration in test solutions of phosphate buffer. Using the spectrophotometric method, 72-h cultures of F. aurantiacum were observed to remove more toxin from solution than 24-h cultures. Likewise, populations of 10(10) cells removed aflatoxin at a faster rate than did 10(9) cells, although the total amount removed did not differ. Transferring F. aurantiacum cultures in tryptic soy broth every 3 days for over 3 days for over 8 months had no apparent effect on their ability to remove measurable amounts of aflatoxin B1 from solution. Populations of 1 X 10(9) CFU/ml or less heat-inactivated F. aurantiacum were unable to remove aflatoxin B1 from phosphate buffer
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X-58.1.91