Regional variations in the number distribution of intrinsic myenteric neurons and coinnervated motor endplates on the striated muscles in the rat esophagus

• Published in: Autonomic neuroscience 2019-07, Vol.219, p.25-32
• Main Authors: Kuramoto, Hirofumi, Yoshimura, Ryoichi, Sakamoto, Hiroshi, Kadowaki, Makoto
• Format: Article
• Language: English
• Subjects: Animals; Choline O-Acetyltransferase - metabolism; Coinnervated motor endplates; Esophagus; Esophagus - anatomy & histology; Esophagus - innervation; Immunohistochemistry; Intrinsic neurons; Male; Motor Endplate - anatomy & histology; Motor Endplate - metabolism; Muscle, Smooth - anatomy & histology; Muscle, Smooth - innervation; Muscle, Striated - anatomy & histology; Muscle, Striated - innervation; Myenteric Plexus - anatomy & histology; Myenteric Plexus - metabolism; Neurons - cytology; Neurons - metabolism; Nitric Oxide Synthase - metabolism; Rats, Wistar; Striated muscles
• ISSN: 1566-0702; 1872-7484
• DOI: 10.1016/j.autneu.2019.03.004

The roles of intrinsic neurons and the significance of the coinnervated striated muscles in the esophagus are unclear. We examined the number distribution of intrinsic neurons and coinnervated motor endplates on the striated muscles in the rat esophagus using immunohistochemistry to investigate whether these neurons and coinnervated striated muscles may be relevant to the local control of esophageal motility. The number of PGP9.5-positive neurons was higher in the cervical esophagus (segment 1) and gradually decreased toward the aboral, with a moderate increase in the abdominal (segment 5). This pattern was similar to that of NOS-positive neurons, while the number of ChAT-positive neurons decreased toward the aboral, but it was not significantly different among segments 3 to 5. The number of ChAT-positive motor endplates increased toward the aboral, with the highest number in segment 5. The proportion of coinnervated motor endplates was approximately 80% in segments 1 to 4, but approximately 66% in segment 5. NPY-IR was localized in some nerve terminals among the smooth muscles of the muscularis mucosa and some NOS- or ChAT-positive esophageal intrinsic neurons. ENK-8-IR was found in some NOS- or ChAT-positive intrinsic neurons, and nerve terminals surrounding intrinsic neurons in the esophagus, but not in motor neurons at the NA or DMV. This study suggests that regional variations in the number of intrinsic neurons and coinnervated striated muscles in the rat esophagus may be involved in local regulations of esophageal motility, and that the rat esophageal intrinsic neurons may contain, at least, motor neurons and interneurons.