Early ovine preantral follicles have a potential to grow until antral stage in two‐step culture system in the presence of aqueous extract of Justicia insularis

The objective of this study was to determine whether preantral follicles cultured in vitro for 7 days within ovine ovarian cortical strips could be isolated at the secondary follicles (SF) and grown until antral stage during an additional 6 days period of in vitro culture in the presence of aqueous...

Full description

Saved in:
Bibliographic Details
Published in:Reproduction in domestic animals 2019-08, Vol.54 (8), p.1121-1130
Main Authors: Mbemya, Gildas Tetaping, Sá, Naiza Arcângela Ribeiro, Guerreiro, Denise Damasceno, Sousa, Francisca Geovania Canafístula, Nguedia, Sylvain Njina, Alves, Benner Geraldo, Santos, Francielli Weber, Pessoa, Otília Deusdênia Loiola, Comizzoli, Pierre, Figueiredo, José Ricardo, Rodrigues, Ana Paula Ribeiro
Format: Article
Language:English
Subjects:
Animals
Antioxidants
antral follicles
Cortex
Culture
Culture Media - chemistry
early secondary follicles
Female
Follicles
Fragments
Gametocytes
in vitro culture
Justicia - chemistry
Justicia insularis
Oocytes
ovarian cortex
Ovarian Follicle - growth & development
Plant Extracts - chemistry
Plant Extracts - pharmacology
Sheep
Tissue Culture Techniques
Trehalose
Trehalose - chemistry
Trehalose - pharmacology
Viability
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The objective of this study was to determine whether preantral follicles cultured in vitro for 7 days within ovine ovarian cortical strips could be isolated at the secondary follicles (SF) and grown until antral stage during an additional 6 days period of in vitro culture in the presence of aqueous extract of Justicia insularis. Fresh ovarian fragments from 16 adult sheep were fixed for histological analysis (Control 1) or in vitro cultured individually in α‐MEM+ supplemented with 0.3 mg/ml J. insularis (Step 1) for 7 days. Part of the fragments then were fixed for histological analysis (in vitro culture group). Remaining fragments were exposed stepwise to increasing trehalose concentrations before immediate isolation of SF and viability assessment (Control 2) or after 6 days of culture in α‐MEM++ supplemented with 0.3 mg/ml J. insularis (Step 2). In Step 1, percentage of follicular activation was 80%. In Step 2, a significant increase (p 
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.13477