A comparison of liquid and lyophilized egg yolk plasma to low density lipoproteins for freezing of canine spermatozoa

The current study aimed to explore the potential usefulness of liquid or lyophilized egg yolk plasma (EYP) as a substitute for low‐density lipoproteins (LDL) for cryopreservation of canine spermatozoa. In the first experiment, a total of 20 ejaculates harvested from six Beagles were frozen in extend...

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Bibliographic Details
Published in:Reproduction in domestic animals 2019-08, Vol.54 (8), p.1131-1138
Main Authors: Belala, Réda, Briand‐Amirat, Lamia, Martinot, Aline, Thorin, Chantal, Michaud, Sandrine, Desherces, Serge, Youngs, Curtis R., Bencharif, Djemil
Format: Article
Language:English
Subjects:
Acridine orange
Animals
canine spermatozoa
Cryopreservation
Cryopreservation - veterinary
Cryoprotective Agents - pharmacology
Density
Deoxyribonucleic acid
DNA
Dogs
Egg Yolk - chemistry
egg yolk plasma
Freeze Drying
Freezing
in vitro fertility tests
Integrity
Iodides
Lipoproteins
Lipoproteins, LDL - pharmacology
Low density lipoprotein
lyophilization
Male
Melting
Motility
Parameters
Propidium iodide
Semen
Semen Analysis - veterinary
Semen Preservation - methods
Semen Preservation - veterinary
Sperm
Sperm Motility - drug effects
Spermatozoa
Spermatozoa - drug effects
Staining
Thawing
Yolk
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Summary:The current study aimed to explore the potential usefulness of liquid or lyophilized egg yolk plasma (EYP) as a substitute for low‐density lipoproteins (LDL) for cryopreservation of canine spermatozoa. In the first experiment, a total of 20 ejaculates harvested from six Beagles were frozen in extenders containing 6% LDL (control) or liquid or lyophilized EYP at one of three concentrations (20%, 40% or 60%). Motility parameters were assessed 10 min after thawing using computer‐assisted sperm analysis. For both liquid and lyophilized EYP, the 40% concentration yielded motility similar (p > 0.05) to that observed with the control extender. In the second experiment, 12 ejaculates collected from the same six dogs were frozen in 6% LDL (Control), 40% liquid EYP or 40% lyophilized EYP extenders. Spermatozoal membrane integrity (hypo‐osmotic swelling test [HOSt] and SYBR14/propidium iodide [PI] staining), acrosome integrity (FITC‐Pisum sativum agglutinin staining) and DNA integrity (acridine orange staining) characteristics were evaluated 10 min after thawing. Both liquid and lyophilized 40% EYP‐based extenders successfully preserved all assessed integrity parameters as efficiently as the control. Results of this study suggest that lyophilized EYP is a viable alternative to LDL in freezing extenders for dog semen.
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.13476