Preferential expression of sialyl 6′-sulfo N-acetyllactosamine-capped O-glycans on high endothelial venules in human peripheral lymph nodes

• Published in: Laboratory investigation 2019-10, Vol.99 (10), p.1428-1441
• Main Authors: Tsutsumiuchi, Manami, Hoshino, Hitomi, Kogami, Akiya, Tsutsumiuchi, Toshiki, Yokoyama, Osamu, Akama, Tomoya O., Kobayashi, Motohiro
• Format: Article
• Language: English
• Subjects: 13/1; 13/109; 13/44; 13/51; 38/77; 38/90; 631/45/221; 631/80/458/1524; 64/60; 82/29; 82/51; Amino Sugars - metabolism; Animals; Antigens, CD34 - metabolism; Carcinoma - metabolism; CD34 antigen; Cell adhesion; Cell adhesion & migration; Cell adhesion molecules; Cell Adhesion Molecules - metabolism; Chains; Endothelial cells; Galactose; Glycan; Glycosaminoglycans; Homing; Humans; Keratan sulfate; L-selectin; Laboratory Medicine; Lymph nodes; Lymph Nodes - metabolism; Lymphatic system; Lymphocytes; Lymphocytes, Tumor-Infiltrating; MAdCAM-1 antigen; Medicine; Medicine & Public Health; Mice, Knockout; Monoclonal antibodies; Mucin; Mucoproteins - metabolism; Mucosa; N-Acetyllactosamine; Organs; Pathology; Polysaccharides; Polysaccharides - metabolism; Sulfates; Sulfation; Sulfotransferase; Venules - metabolism
• ISSN: 0023-6837; 1530-0307
• DOI: 10.1038/s41374-019-0267-0

Lymphocyte “homing”, the physiologic trafficking of lymphocytes from the circulation to secondary lymphoid organs, is regulated by sequential adhesive interactions between lymphocytes and endothelial cells that constitute high endothelial venules (HEVs). Initial lymphocyte “rolling” is mediated by relatively weak, transient adhesive interactions between L-selectin expressed on lymphocytes and sulfated mucin-type O-glycans expressed on HEVs. Keratan sulfate galactose (Gal)-6-O-sulfotransferase (KSGal6ST) catalyzes 6-O-sulfation of Gal in keratan sulfate glycosaminoglycan chains but also transfers sulfate to Gal in much shorter glycan chains, such as sialylated N-acetyllactosamine (LacNAc)-capped O-glycans. In mice, KSGal6ST is reportedly expressed in HEVs and functions in synthesizing 6-sulfo Gal-containing O-glycans on HEVs. However, in humans, the presence of 6-sulfo Gal-containing O-glycans on HEVs is not reported. Employing the newly developed monoclonal antibody 297-11A, which recognizes non-sialylated terminal 6′-sulfo LacNAc, we demonstrate that sialyl 6′-sulfo (and/or 6,6′-disulfo) LacNAc-capped O-glycans are preferentially displayed on HEVs in human peripheral lymph nodes (PLNs) and to a lesser extent in mesenteric LNs (MLNs) but not in Peyer's patches (PPs). We also found that the scaffold protein mucosal addressin cell adhesion molecule 1 (MAdCAM-1), which is expressed on HEVs in PPs and MLNs but not PLNs, was modified by 297-11A-positive sulfated glycans less efficiently than was CD34. Moreover, 297-11A-positive sulfated glycans were also displayed on HEV-like vessels induced in tumor-infiltrating lymphocyte (TIL) aggregates formed in various cancers. These findings collectively indicate that 297-11A-positive sulfated glycans potentially play a role in physiologic lymphocyte homing as well as in lymphocyte recruitment under pathologic conditions.