Principles for Integrative Structural Biology Studies

• Published in: Cell 2019-05, Vol.177 (6), p.1384-1403
• Main Authors: Rout, Michael P., Sali, Andrej
• Format: Article
• Language: English
• Subjects: Algorithms; Animals; Computational Biology - methods; Humans; Models, Molecular; Molecular Biology; Nuclear Pore - physiology; Software; Systems Analysis; Systems Biology - methods; Systems Integration
• ISSN: 0092-8674; 1097-4172
• DOI: 10.1016/j.cell.2019.05.016

Integrative structure determination is a powerful approach to modeling the structures of biological systems based on data produced by multiple experimental and theoretical methods, with implications for our understanding of cellular biology and drug discovery. This Primer introduces the theory and methods of integrative approaches, emphasizing the kinds of data that can be effectively included in developing models and using the nuclear pore complex as an example to illustrate the practice and challenges involved. These guidelines are intended to aid the researcher in understanding and applying integrative structural methods to systems of their interest and thus take advantage of this rapidly evolving field. Integrative structure determination is a powerful approach to modeling the structures of biological systems based on data produced by multiple experimental and theoretical methods, with implications for our understanding of cellular biology and drug discovery. This Primer introduces the theory and methods of integrative approaches, emphasizing the kinds of data that can be effectively included in developing models and using the nuclear pore complex as an example to illustrate the practice and challenges involved. These guidelines are intended to aid the researcher in understanding and applying integrative structural methods to systems of their interest and thus take advantage of this rapidly evolving field.