Porphyrin‑silver nanoparticles hybrids: Synthesis, characterization and antibacterial activity

The preparation of light-activated hybrid antibacterial agent combining the porphyrin molecules, bound to the silver nanoparticles (AgNPs) surface is reported. AgNPs were synthesized by N-methyl-2-pyrrolidone-initiated reduction without additional reducing agents. The chemical structure of protoporp...

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Bibliographic Details
Published in:Materials Science & Engineering C 2019-09, Vol.102, p.192-199
Main Authors: Elashnikov, R., Radocha, M., Panov, I., Rimpelova, S., Ulbrich, P., Michalcova, A., Svorcik, V., Lyutakov, O.
Format: Article
Language:English
Subjects:
Antibacterial
Antibacterial activity
Antibacterial materials
E coli
Energy transmission
Fluorescence
Hybrids
Infrared spectroscopy
Light emitting diodes
Light-triggered
Materials science
N-Methyl-2-pyrrolidone
Nanoparticles
Organic chemistry
Porphyrin
Protoporphyrin
Protoporphyrin IX
Raman spectroscopy
Reagents
Reducing agents
Silver
Silver nanoparticles
Size distribution
Spectroscopy
Spectrum analysis
Transmission electron microscopy
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Summary:The preparation of light-activated hybrid antibacterial agent combining the porphyrin molecules, bound to the silver nanoparticles (AgNPs) surface is reported. AgNPs were synthesized by N-methyl-2-pyrrolidone-initiated reduction without additional reducing agents. The chemical structure of protoporphyrin IX was modified with the aim to introduce thiol groups. The size distribution and shape features of AgNPs were checked using TEM and HRTEM microscopies. The introduction of thiol groups into the porphyrin was proved by IR spectroscopy. The AgNPs-porphyrin binding was performed in solution and confirmed by fluorescence quenching, Raman spectroscopy and energy-filtered transmission electron microscopy (EFTEM). The antibacterial tests were performed against S. epidermidis and E. coli upon to LED illumination and in the dark. The synergetic effect of AgNPs and porphyrin as well as light activation of the created antibacterial conjugates were observed. •The light-activated hybrid antibacterial agent combining the porphyrin and AgNPs is reported.•The chemical structure of porphyrin was modified for introduction of thiol groups, responsible for grafting to AgNPs•The antibacterial activity of prepared conjugates was tested on gram-negative and gram-positive bacterial strains.•The synergetic effect of AgNPs and porphyrin under illuminatwas observed.•The prepared hybrids show better antibacterial activity then the pristine porphyrin and AgNPs.
ISSN:0928-4931
1873-0191
DOI:10.1016/j.msec.2019.04.029