An electrochemical aptasensing platform for carbohydrate antigen 125 based on the use of flower-like gold nanostructures and target-triggered strand displacement amplification

An electrochemical aptasensing method is described for the determination of the biomarker CA125. It combines aptamer recognition and target-triggered strand displacement amplification. Flower like gold nanostructures were electrodeposited on a screen-printed carbon electrode to increase the sensor s...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2019-06, Vol.186 (6), p.388-388, Article 388
Main Authors: Chen, Jishun, Hu, Wanbao, Wei, Jian, Yu, Fei, Wu, Lun, Wang, Ceming, Wang, Wei, Zuo, Shenyuan, Shang, Bing, Chen, Qinhua
Format: Article
Language:English
Subjects:
Analytical Chemistry
Antigens
Aptamers, Nucleotide - chemistry
Aptamers, Nucleotide - genetics
Biosensing Techniques - methods
CA-125 Antigen - analysis
CA-125 Antigen - blood
CA-125 Antigen - urine
Carbon - chemistry
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
DNA - chemistry
DNA - genetics
DNA Probes - chemistry
DNA Probes - genetics
Electric properties
Electrochemical Techniques - instrumentation
Electrochemical Techniques - methods
Electrodes
Gold - chemistry
Humans
Limit of Detection
Membrane Proteins - analysis
Membrane Proteins - blood
Membrane Proteins - urine
Metal Nanoparticles - chemistry
Methylene blue
Microengineering
Nanochemistry
Nanotechnology
Nucleic Acid Amplification Techniques - methods
Nucleic Acid Hybridization
Original Paper
Saliva - chemistry
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Summary:An electrochemical aptasensing method is described for the determination of the biomarker CA125. It combines aptamer recognition and target-triggered strand displacement amplification. Flower like gold nanostructures were electrodeposited on a screen-printed carbon electrode to increase the sensor surface, to assemble more toehold-containing hairpin probe 1 (Hp1), and to improve the accessibility for DNA strands. Under the optimal conditions, this assay has a linear response in the 0.05 to 50 ng•mL −1 CA125 concentration range, with a low detection limit of 5.0 pg•mL −1 . This method is specific and stable. It was successfully applied to the detection of CA125 in spiked biological samples, with recoveries between 82.5% and 104.1%. Graphical abstract
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-019-3497-3