Systemic and local effects of radiotherapy: an experimental study on implants placed in rats

Objectives Evaluate the modulating effect of ionizing radiation, blood cytokine levels, and bone remodeling of the interface around the implant to understand the radiation mechanisms which can impair the implants receptor site. Material and methods Sixty rats were submitted to grade V titanium impla...

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Bibliographic Details
Published in:Clinical oral investigations 2020-02, Vol.24 (2), p.785-797
Main Authors: da Cruz Vegian, Mariana Raquel, Costa, Bruno César Almeida, de Fátima Santana-Melo, Gabriela, Godoi, Fernanda Herrera Costa, Kaminagakura, Estela, Tango, Rubens Nisie, do Prado, Renata Falchete, de Oliveira, Luciane Dias, Federico, Claudio Antonio, de Oliveira Marco Avelino, Sarah, Neves, Rafael Marques, de Vasconcellos, Luana Marotta Reis
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Bone growth
Bone remodeling
Caspase
Caspase-3
Dental Implants
Dentistry
Enzyme-linked immunosorbent assay
Femur
I.R. radiation
IL-1β
Implants, Experimental
Inflammation
Interleukin 10
Interleukin 6
Ionizing radiation
Macrophage colony-stimulating factor
Medicine
Original Article
Osseointegration
Osteocalcin
Osteogenesis
Radiation therapy
Rats
Serum levels
Statistical analysis
Surgery
Titanium
Transplants & implants
Tumor necrosis factor
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Summary:Objectives Evaluate the modulating effect of ionizing radiation, blood cytokine levels, and bone remodeling of the interface around the implant to understand the radiation mechanisms which can impair the implants receptor site. Material and methods Sixty rats were submitted to grade V titanium implants in the femurs and were divided into the following groups: no-irradiation (N-Ir): control group with implant only; early-irradiation (E-Ir): implant + irradiation after 24 h; late-irradiation (L-Ir): implant + irradiation after 4 weeks; and previous-irradiation (P-Ir): irradiation + implant after 4 weeks. The animals in the E-Ir, L-Ir, and P-Ir groups were irradiated in two fractional stages of 15 Gy. At 3 days, 2 weeks, and 7 weeks after the final procedure, five animals were randomly euthanized per group. Serum levels of TNF-ɑ, IL-1β, TGF-β, IL-6, M-CSF, and IL-10 were measured from blood collected prior to euthanasia using the ELISA test. The pieces containing the implants were subjected to immunohistochemical labeling using the tartrate acid resistant to phosphatase, osteocalcin, and caspase-3 markers and mCT. The ANOVA test was used for statistical analysis, and the Tukey multiple comparison test ( p  
ISSN:1432-6981
1436-3771
DOI:10.1007/s00784-019-02946-5