Fast Determination of Main Bioamines and Precursor Amino Acids in Beer by Miniaturized Electrophoresis Using Gold Nanoparticle Composite Electrode

• Published in: Food analytical methods 2019-03, Vol.12 (3), p.644-651
• Main Authors: Luo, Dan, Zhao, Xiaoshuang, Zheng, Yiliang, Ma, Yaolu, Cun, Fei, Ye, Jiannong, Chu, Qingcui
• Format: Article
• Language: English
• Subjects: Amino acids; Analytical Chemistry; beers; Capillary electrophoresis; chemical species; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; electric potential difference; Electrical measurement; Electrodes; Electrophoresis; energy-dispersive X-ray analysis; Food analysis; Food safety; Food Science; Fourier transform infrared spectroscopy; Fourier transforms; Gold; Graphene; graphene oxide; Infrared analysis; Infrared spectroscopy; Microbiology; Morphology; nanogold; Nanoparticles; Precursors; rapid methods; Reproducibility; Scanning electron microscopy; Sodium hydroxide; voltammetry; X-ray diffraction
• ISSN: 1936-9751; 1936-976X
• DOI: 10.1007/s12161-018-1395-7

A gold nanoparticle/reduced graphene oxide composite electrode (AuNP/rGO) was fabricated for the determination of the main bioamines and their precursor amino acids in beer based on a miniaturized capillary electrophoresis with amperometric detection system (mini-CE-AD). The surface morphology and structure of the synthetic AuNP/rGO material were observed by scanning electron microscope, energy dispersive X-ray spectroscope, X-ray diffraction, and Fourier transform infrared spectroscopy. The analytical performance of the composite electrode was verified by cyclic voltammetry and mini-CE-AD approaches. Four target analytes were separated within 7 min in 50 mM Na 2 B 4 O 7 -NaOH buffer (pH 9.35) at a separation voltage of 3 kV. This developed method has been applied to the beer samples, and acceptable recovery could be obtained ranging from 84.5 to 108%. The assay results indicated that this AuNP/rGO composite electrode has the advantages of simple preparation, low working potential (+ 0.65 V vs. SCE), good sensitivity (0.13~0.87 μM), and satisfactory reproducibility. This proposed method provides an alternative for rapid analysis of food safety.