Anti-proliferative and anti-apoptotic potential effects of epigallocatechin-3-gallate and/or metformin on hepatocellular carcinoma cells: in vitro study

The effects of epigallocatechin-3-gallate (EGCG) and metformin single treatment have been tested against hepatocellular carcinoma (HCC). This study aimed to assess the combination effects of EGCG and metformin on proliferation and apoptosis of HepG2cells and identified new potential molecular target...

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Bibliographic Details
Published in:Molecular biology reports 2019-04, Vol.46 (2), p.2039-2047
Main Authors: Sabry, Dina, Abdelaleem, Omayma O., El Amin Ali, Amani M., Mohammed, Rehab A., Abdel-Hameed, Nehal D., Hassouna, Amira, Khalifa, Warda A.
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Antidiabetics
Apoptosis
Biomedical and Life Sciences
Caspase
Caspase-3
Cell proliferation
Cyclin D1
cyclins
Epigallocatechin gallate
Gene expression
Heparan sulfate proteoglycans
Hepatocellular carcinoma
hepatoma
Histology
Life Sciences
Liver cancer
Metformin
Morphology
Original Article
Polymerase chain reaction
pro-apoptotic proteins
protein synthesis
reverse transcriptase polymerase chain reaction
Reverse transcription
Survivin
toxicity testing
Vascular endothelial growth factor
Western blotting
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Summary:The effects of epigallocatechin-3-gallate (EGCG) and metformin single treatment have been tested against hepatocellular carcinoma (HCC). This study aimed to assess the combination effects of EGCG and metformin on proliferation and apoptosis of HepG2cells and identified new potential molecular targets. The effect of EGCG and metformin against cell proliferation in HepG2 was determined using MTT assay. Reverse transcription polymerase chain reaction was applied to examine the gene expression of cyclin D1, lncRNA-AF085935, caspase-3, survivin and VEGF. The level of protein expression of glypican-3 was assessed by western blot. In HepG2 cells, EGCG and metformin combination treatment exhibited high significant effect against tumor proliferation. It significantly reduced cyclin D1, lncRNA-AF085935, glypican-3 and promoted apoptosis through increasing caspase3 and decreasing survivin compared to control cells. Moreover, EGCG and metformin treated cells showed decreased expression levels of VEGF. Our study provided new insights of the anticarcinogenic effects of EGCG and metformin on HCC through their effects on glypican-3 and lncRNA-AF085935.
ISSN:0301-4851
1573-4978
DOI:10.1007/s11033-019-04653-6