Surface-layer protein from Lactobacillus acidophilus NCFM attenuates tumor necrosis factor-α-induced intestinal barrier dysfunction and inflammation

Lactobacillus acidophilus NCFM, a probiotic generally regarded as safe, carries a proteinaceous surface (S) layer, composed of numerous identical subunits (surface layer protein, Slp). S-layer proteins have been confirmed to possess multiple biological properties, but their role in maintaining the i...

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Bibliographic Details
Published in:International journal of biological macromolecules 2019-09, Vol.136, p.27-34
Main Authors: Wang, Huifang, Zhang, Qiuxiang, Niu, Yuhan, Zhang, Xin, Lu, Rongrong
Format: Article
Language:English
Subjects:
Active Transport, Cell Nucleus - drug effects
Apoptosis - drug effects
Bacterial Proteins - pharmacology
Bacterial Proteins - therapeutic use
Caco-2 cell monolayers
Caco-2 Cells
Cell Nucleus - drug effects
Cell Nucleus - metabolism
Cell Survival - drug effects
Gene Expression Regulation - drug effects
Humans
Inflammation
Inflammation - drug therapy
Interleukin-8 - metabolism
Intestinal epithelial barrier
Intestinal Mucosa - cytology
Intestinal Mucosa - drug effects
Intestinal Mucosa - metabolism
L. acidophilus NCFM
Lactobacillus acidophilus
Membrane Glycoproteins - pharmacology
Membrane Glycoproteins - therapeutic use
Permeability - drug effects
Surface-layer protein
Tight Junction Proteins - metabolism
Transcription Factor RelA - metabolism
Tumor Necrosis Factor-alpha - pharmacology
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Summary:Lactobacillus acidophilus NCFM, a probiotic generally regarded as safe, carries a proteinaceous surface (S) layer, composed of numerous identical subunits (surface layer protein, Slp). S-layer proteins have been confirmed to possess multiple biological properties, but their role in maintaining the intestinal epithelial barrier is not fully known. We investigated the effects of Slp on tumor necrosis factor (TNF)-α-elicited intestinal barrier dysfunction and explored the underlying molecular mechanism. TNF-α administration markedly induced intestinal epithelial injury and inflammation in Caco-2 cells. Preincubation of Caco-2 cells with Slp at concentrations ranging from 50 to 100 μg/mL for 6 h improved intestinal epithelial cell integrity and permeability, restored ZO-1 and Occludin protein expressions (P 
ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2019.06.041