Endo-siRNAs repress expression of SINE1B during in vitro maturation of porcine oocyte

Approximately 40% of mammalian genome is made of transposable elements (TEs), and during specific biological processes, such as gametogenesis, they may be activated by global demethylation, so strict silencing mechanism is indispensable for genomic stability. Here, we performed small RNA-seq on Dice...

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Published in:Theriogenology 2019-09, Vol.135, p.19-24
Main Authors: Kong, Qing-ran, Zhang, Jia-ming, Zhang, Xiao-lei, Zong, Ming, Zheng, Kai-lun, Liu, Lei, Meng, Fan-xi, Zhang, Ting-ting, Weng, Xiao-gang, Mu, Yan-shuang, Liu, Zhong-hua
Format: Article
Language:English
Subjects:
Animals
DEAD-box RNA Helicases - genetics
DEAD-box RNA Helicases - metabolism
DNA Methylation
endo-siRNAs
Gene Expression Regulation
Gene Knockdown Techniques
In Vitro Oocyte Maturation Techniques - veterinary
IVM
Oocyte
Oocytes - physiology
Pig
Retroelements
RNA, Small Interfering
Short Interspersed Nucleotide Elements - genetics
Short Interspersed Nucleotide Elements - physiology
SINE1B
Swine
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Summary:Approximately 40% of mammalian genome is made of transposable elements (TEs), and during specific biological processes, such as gametogenesis, they may be activated by global demethylation, so strict silencing mechanism is indispensable for genomic stability. Here, we performed small RNA-seq on Dicer1 knockdown (KD) oocytes in pig, and observed short interspersed nuclear elements 1B (SINE1B) derived endogenous small interfering RNAs (endo-siRNAs), termed SINE1B-siRNAs, were significantly decreased and their biogenesis was dependent on Dicer1 and transcript of SINE1B. Furthermore, by injection of mimics and inhibitors of the SINE1B-siRNAs into germinal vesicle-stage (GV-stage) oocytes, we found the maturation rate was significantly decreased by SINE1B-siRNAs, indicating the SINE1B-siRNAs are indispensible for in vitro maturation (IVM) of porcine oocyte. To figure out the mechanism, we checked the expression pattern and DNA methylation status of SINE1B during IVM of porcine oocytes, and demonstrated the SINE1B-siRNAs could repress SINE1B expression induced by hypomethylation at a post-transcriptional level. Our results suggest that during gametogenesis when the erasure of DNA methylation occurs, endo-siRNAs act as a chronic response to limit retrotransposon activation. •The biogenesis of SINE1B-siRNAs is Dicer1 and SINE1B transcripts dependent.•SINE1B-siRNAs are benefit for in vitro maturation of porcine oocyte.•SINE1B-siRNAs can repress SINE1B expression at a post-transcriptional level.
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2019.05.011