Higher functionality of bacterial plasmid DNA in water after peracetic acid disinfection compared with chlorination

• Published in: The Science of the total environment 2019-10, Vol.685, p.419-427
• Main Authors: Zhang, Chiqian, Brown, Pamela J.B., Hu, Zhiqiang
• Format: Article
• Language: English
• Subjects: Antibiotic resistance gene; Chlorine; Disinfection; Functionality; Peracetic acid; Plasmid DNA
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2019.05.074

Peracetic acid (PAA) is an emerging disinfectant with a low disinfection by-product formation potential, but how PAA destroys gene function after killing bacteria remains to be studied. Bacterial plasmid DNA is a mobile genetic element that often harbors undesirable genes encoding antibiotic resistance and virulence factors. Even though PAA efficiently kills bacteria, bacterial plasmids and other mobile genetic elements might still be intact and functional after PAA disinfection, posing potential public health and environmental risks. This study evaluated the impact of PAA disinfection on the functionality of plasmid DNA in vivo and compared the results with those from chlorination. We delivered a plasmid DNA harboring two antibiotic resistance genes to Escherichia coli TOP10 to form an antibiotic-resistant bacterium (ARB). The planktonic ARB was treated with PAA and chlorine to find the minimum doses inhibiting the regrowth of the strain. PAA and chlorine stopped the regrowth at 8 ± 1 mg PAA·L−1 and 20 ± 9 mg Cl2·L−1, respectively. The functionality of the plasmid DNA after PAA and chlorine disinfection was then determined at higher doses in vivo. Neither PAA nor chlorine completely destroyed the plasmid DNA. However, chlorine was more efficient than PAA in eliminating the plasmid DNA. PAA at 25 mg PAA·L−1 reduced the transforming activity of the plasmid DNA by less than 0.3 log10 units, whereas chlorine at 25 mg Cl2·L−1 reduced the transforming activity by approximately 1.7 log10 units. Chlorine had a more pronounced impact on the functionality of the plasmid DNA because it oxidizes or destroys bacterial components including plasmid DNA faster than PAA. In addition, environmental scanning electron microscopy shows that chlorination desiccated the cells resulting in the flat cellular structure and possibly more complete loss of plasmid DNA, whereas PAA disinfection had a less impact on cell structure and morphology. This study demonstrates that more plasmid DNA remains functional in water after PAA disinfection than after chlorination. These functional genetic elements could be acquired by other microorganisms via horizontal gene transfer to pose potential public health and environmental risks. [Display omitted] •Functionality of plasmid DNA was investigated in vivo after peracetic acid (PAA) disinfection.•PAA was more efficient than chlorine in preventing bacterial regrowth.•Neither PAA nor chlorine completely destroyed plasmid while preventing bacterial