Detection of Bacillus cereus diarrheal enterotoxin in raw and pasteurized milk

Raw and pasteurized milk samples submitted for routine quality analysis were screened for the presence of Bacillus cereus diarrheal enterotoxin (BDE) using the TECRA BDE Visual Immunoassay (VIA) kit. BDE was not detected in 298 raw milk samples tested by the TECRA VIA. B. cereus was isolated from 2...

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Bibliographic Details
Published in:Journal of food protection 1997-11, Vol.60 (11), p.1391-1393
Main Authors: Odumeru, J.A. (Ontario Ministry of Agriculture, Food and Rural Affairs, Guelph, Ontario, Canada.), Toner, A.K, Muckle, C.A, Griffiths, M.W, Lynch, J.A
Format: Article
Language:English
Subjects:
BACILLUS CEREUS
Biological and medical sciences
BIOLOGICAL CONTAMINATION
CONTAMINACION BIOLOGICA
CONTAMINATION BIOLOGIQUE
Food industries
Fundamental and applied biological sciences. Psychology
LAIT
LAIT PASTEURISE
LECHE
LECHE PASTEURIZADA
MICROBIAL CONTAMINATION
MILK
Milk and cheese industries. Ice creams
PASTEURIZED MILK
RAW MILK
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Summary:Raw and pasteurized milk samples submitted for routine quality analysis were screened for the presence of Bacillus cereus diarrheal enterotoxin (BDE) using the TECRA BDE Visual Immunoassay (VIA) kit. BDE was not detected in 298 raw milk samples tested by the TECRA VIA. B. cereus was isolated from 2 of 298 (0.7%) raw milk samples cultured. Culture supernatants from these isolates were positive for BDE in the TECRA VIA but negative in the Reverse Passive Latex Agglutination (RPLA) test for BDE. Forty-three of 112 (38.4%) pasteurized milk samples incubated at 10 degrees C until their expiry dates were positive for BDE by the TECRA VIA. The same number of samples incubated at 4 degrees C had no detectable levels of enterotoxin. B. cereus in the range of 10(3) to 10(6) CFU/ml was isolated from all BDE-positive pasteurized milk samples. BDE was detected in the culture supernatants of all the 43 isolates by TECRA VIA and in 30 of these isolates by RPLA. These results demonstrate that moderate temperature abuse of pasteurized milk may allow the growth of B. cereus and BDE production
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X-60.11.1391