Gold nanoparticles regulate tight junctions and improve cetuximab effect in colon cancer cells

Colon cancer (CC) is the second cause of cancer death worldwide. The use of nanoparticles for drug delivery has been increasing in cancer clinical trials over recent years. We evaluated cytotoxicity of citrate-capped gold nanoparticles (GNPs) and the role they play on cell–cell adhesion. We also use...

Full description

Saved in:
Bibliographic Details
Published in:Nanomedicine (London, England) England), 2019-06, Vol.14 (12), p.1565-1578
Main Authors: Leve, Fernanda, Bonfim, Daniella P, Fontes, Giselle, Morgado-Díaz, José A
Format: Article
Language:English
Subjects:
Autophagy
Cancer therapies
Cell adhesion & migration
cell–cell adhesion
cetuximab
colon cancer
Colorectal cancer
Cytotoxicity
Drug delivery systems
EGFR
gold nanoparticle
Immunoglobulins
Immunotherapy
Investigations
Lung cancer
Monoclonal antibodies
Mutation
Nanoparticles
Permeability
Physiology
Proteins
Regulatory approval
Targeted cancer therapy
tight junction
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Colon cancer (CC) is the second cause of cancer death worldwide. The use of nanoparticles for drug delivery has been increasing in cancer clinical trials over recent years. We evaluated cytotoxicity of citrate-capped gold nanoparticles (GNPs) and the role they play on cell–cell adhesion. We also used GNP for delivery of cetuximab into different CC cell lines. CC cells with well-formed tight junctions impair GNP uptake. Noncytotoxic concentration of GNP increases paracellular permeability in Caco-2 cells in a reversible way, concomitantly to tight junctions proteins CLDN1 and ZO-1 redistribution. GNP functionalized with cetuximab increases death of invasive HCT-116 CC cells. GNP can be used for drug delivery and can improve efficiency of CC therapy.
ISSN:1743-5889
1748-6963
DOI:10.2217/nnm-2019-0023