Circulating tumor cell-related transcripts in blood as prognostic biomarkers of early recurrence after liver resection for colorectal metastases

Background: Several prognostic factors were proposed to improve early detection of recurrence after liver resection of metastases of colorectal cancer. Circulating tumor cell-related transcripts were evaluated in colorectal cancer patients with conflicting results. The aim of this study was to inves...

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Bibliographic Details
Published in:The International journal of biological markers 2019-09, Vol.34 (3), p.269-275
Main Authors: Giuliante, Felice, Panettieri, Elena, Ardito, Francesco, De Rose, Agostino, Pocino, Krizia, Autilio, Chiara, Paolillo, Carmela, Capoluongo, Ettore Domenico
Format: Article
Language:English
Subjects:
Adult
Antigens
Biomarkers
Biomarkers, Tumor - blood
Carcinoembryonic antigen
Clinical trials
Colorectal cancer
Colorectal carcinoma
Colorectal Neoplasms - secondary
Copy number
Epidermal growth factor
ERCC1 protein
Female
Glyceraldehyde-3-phosphate dehydrogenase
Hepatectomy
Humans
Liver
Liver - pathology
Liver - surgery
Liver cancer
Liver Neoplasms - blood
Male
Medical prognosis
Metastases
Metastasis
Middle Aged
Neoplasm Metastasis
Neoplasm Recurrence, Local - blood
Patients
Peripheral blood
Prognosis
Reverse transcription
Surgery
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Summary:Background: Several prognostic factors were proposed to improve early detection of recurrence after liver resection of metastases of colorectal cancer. Circulating tumor cell-related transcripts were evaluated in colorectal cancer patients with conflicting results. The aim of this study was to investigate usefulness of carcinoembryonic antigen CAM5, epidermal growth factor receptor, and ERCC1 transcripts in the bloodstream as predictive factors of recurrence in patients who underwent liver resection for metastases of colorectal cancer. Methods: Peripheral blood was collected from 29 patients at the time of the colorectal cancer liver metastasis resection, and from 25 normal controls. Follow-up draws (FUDs) were also performed at 30 days, and 3 and 12 months since surgery. On each sample, carcinoembryonic antigen CAM5, ERCC1, and GAPDH mRNAs were examined by quantitative reverse transcription (qRT). Results: Carcinoembryonic antigen transcript levels were linearly correlated to the number of spiked cells (qRT analytical limit = five cells). Among 29 patients (20 M/9 F; mean age 63 years (range 32–79), highly significant levels of carcinoembryonic antigen, if compared to the baseline, were detected in those relapsing after surgery (P
ISSN:1724-6008
0393-6155
1724-6008
DOI:10.1177/1724600819849438