Multiparametric Time-Correlated Single Photon Counting Luminescence Microscopy

Classic time-correlated single photon counting (TCSPC) technique involves detection of single photons of a periodic optical signal, registration of the photon arrival time in respect to the reference pulse, and construction of photon distribution with regard to the detection times. This technique ac...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2019, Vol.84 (Suppl 1), p.51-68
Main Authors: Shcheslavskiy, V. I., Shirmanova, M. V., Jelzow, A., Becker, W.
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Detection times
Fluorescence
Life Sciences
Microbiology
Microscope and microscopy
Microscopy
Microscopy, Fluorescence - methods
Optical communication
Optical Imaging - methods
Parameters
Photons
Physiological aspects
Quantum theory
Review
Time correlation functions
Time Factors
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Summary:Classic time-correlated single photon counting (TCSPC) technique involves detection of single photons of a periodic optical signal, registration of the photon arrival time in respect to the reference pulse, and construction of photon distribution with regard to the detection times. This technique achieves extremely high time resolution and near-ideal detection efficiency. Modern TCSPC is multi-dimensional, i.e., in addition to the photon arrival time relative to the excitation pulse, spatial coordinates within the image area, wavelength, time from the start of the experiment, and many other parameters are determined for each photon. Hence, the multi-dimensional TCSPC allows generation of photon distributions over these parameters. This review describes both classic and multi-dimensional types of TCSPC microscopy and their application for fluorescence lifetime imaging in different areas of biological studies.
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297919140049