Growth and photosynthetic activity of Chlamydomonas reinhardtii entrapped in lens-shaped silica hydrogels

• Published in: Journal of biotechnology 2019-08, Vol.302, p.58-66
• Main Authors: Homburg, Sarah Vanessa, Kruse, Olaf, Patel, Anant V.
• Format: Article
• Language: English
• Subjects: Chlamydomonas; Chlamydomonas reinhardtii - growth & development; Chlamydomonas reinhardtii - metabolism; Entrapment; Hydrogels - chemistry; Immobilisation; Microalgae; Microalgae - metabolism; Microalgae - physiology; Photosynthesis - physiology; Silica; Silicon Dioxide; Sol-gel
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/j.jbiotec.2019.06.009

•Entrapment of cells in low-sodium hydrogel lenses maintained growth and photosynthetic activity.•Particle size reduction increases light supply, but maintains the specific growth rate in the silica hydrogel lenses.•Elevated biomass loading of gels leads to increased photosynthetic growth.•Proof-of-concept revealed hydrogen production by cells entrapped in low-sodium silica lenses. Entrapment of microalgae in silica hydrogels enables the application as biocatalysts in continuous production of secreted products. Despite a mitigation of substrate and product diffusion limitations by lens-shaped particles, there are no reports on light supply and limitation. This study investigated the impact of hydrogel structure, particle size and biomass loading on the behaviour of the microalga Chlamydomonas reinhardtii entrapped in lens-shaped silica particles. Entrapment in tetraethyl orthosilicate and tetra(n-propylamino)silane based hydrogels reduced the growth rate by 30% and 23%, respectively. In contrast, cells entrapped in sodium silicate based hydrogels displayed a growth rate similar to free cells and cells entrapped in calcium alginate (1.13 d−1), indicating a suitable biocompatibility. Reduction of lens height by 26% maintained the growth rate in silica hydrogel. A fourfold increase in biomass loading reduced the growth rate by 20% and elevated the yield coefficient by 211%, indicating the impact of biomass loading on light and nutrient supply on photosynthetic growth. Finally, hydrogen production was observed by entrapped cells. The results of this work will pave the way for robust biocatalytic processes where photosynthetically active cells are protected against harmful mechanical and biological influences.