Kinetic Measurements of Singlet Oxygen Phosphorescence in Hydrogen-Free Solvents by Time-Resolved Photon Counting

Solvents lacking hydrogen atoms are very convenient models for elucidating the properties of singlet oxygen, since the lifetime of singlet oxygen in these solvents reaches tens milliseconds. Measuring intrinsic infrared (IR) phosphorescence of singlet oxygen at 1270 nm is the most reliable method of...

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Bibliographic Details
Published in:Biochemistry (Moscow) 2019-02, Vol.84 (2), p.153-163
Main Authors: Krasnovsky, A. A., Benditkis, A. S., Kozlov, A. S.
Format: Article
Language:English
Subjects:
Absorption
Absorption spectra
Absorptivity
Biochemistry
Biomedical and Life Sciences
Biomedicine
Bioorganic Chemistry
Carbon tetrachloride
Chemical properties
Data processing
Excitation
Hydrogen atoms
Irradiance
Life Sciences
Measurement
Microbiology
Optical properties
Oxygen
Phosphorescence
Photochemistry
Photomultiplier tubes
Photon detectors
Rate constants
Singlet oxygen
Solvents
Steady state
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Summary:Solvents lacking hydrogen atoms are very convenient models for elucidating the properties of singlet oxygen, since the lifetime of singlet oxygen in these solvents reaches tens milliseconds. Measuring intrinsic infrared (IR) phosphorescence of singlet oxygen at 1270 nm is the most reliable method of singlet oxygen detection. However, efficient application of the phosphorescence method to these models requires an equipment allowing reliable measurement of the phosphorescence kinetic parameters in the millisecond time range at low rates of singlet oxygen generation, which is a technically difficult problem. Here, we describe a highly sensitive LED (laser) spectrometer recently constructed in our laboratory for the steady-state and time-resolved measurements of the millisecond phosphorescence of singlet oxygen. In the steady-state mode, this spectrometer allows detection of singlet oxygen phosphorescence upon direct excitation of oxygen molecules in the region of dark-red absorption bands at 690 and 765 nm. For kinetic measurements, we used phenalenone as a photo-sensitizer, microsecond pulses of violet (405 nm) LED for excitation (irradiance intensity,
ISSN:0006-2979
1608-3040
DOI:10.1134/S0006297919020068