Fucoidans from brown algae Laminaria longipes and Saccharina cichorioides: Structural characteristics, anticancer and radiosensitizing activity in vitro

• Published in: Carbohydrate polymers 2019-10, Vol.221, p.157-165
• Main Authors: Usoltseva, Roza V., Shevchenko, Natalia M., Malyarenko, Olesya S., Anastyuk, Stanislav D., Kasprik, Anna E., Zvyagintsev, Nikolay V., Ermakova, Svetlana P.
• Format: Article
• Language: English
• Subjects: Anticancer activity; Antineoplastic Agents - chemistry; Antineoplastic Agents - pharmacology; Carbohydrate Sequence; Cell Line, Tumor; Fucoidan; Humans; Laminaria; Laminaria - chemistry; Polysaccharides - chemistry; Polysaccharides - pharmacology; Radiation-Sensitizing Agents - chemistry; Radiation-Sensitizing Agents - pharmacology; Radiosensitizing effect; Saccharina
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2019.05.079

•Fucoidan LlF from L. longipes was α-L-fucan with unusual structure.•LlF consisted of 1,2-, 1,3- and 1,4-linked α-L-Fuc residues.•Fucans LlF and ScF had anticancer effect on melanoma and colon cancer cells.•Fucans LlF and ScF possessed comparable radiosensitizing activity. The sulfated α-l-fucans ScF and LlF were obtained from brown algae of the Laminariaceae family (Saccharina cichorioides and Laminaria longipes). According to spectroscopy NMR, the LlF fucan predominantly contained the →3)-α-l-Fucp-(2SO3–)-(1→4)-α-l-Fucp-(1→2)-α-l-Fucp-(4SO3–)-(1→ repeating units, with small amounts of disaccharide 1,4-linked fragments and 3-sulfated fucose residues. Mass spectrometric analysis revealed the presence of the following fragments in the fucan structure: α-l-Fucp-(2SO3–)-(1→4)-α-l-Fucp-(2SO3–)-(1→3)-α-l-Fucp-(4SO3–); α-l-Fucp-(2,4SO3–)-(1→3)-α-l-Fucp-(1→3)-α-l-Fucp-(4SO3–); α-l-Fucp-(2SO3–)-(1→2)-α-l-Fucp; α-l-Fucp-(2SO3–)-(1→2)-α-l-Fucp-(4SO3–); α-l-Fucp-(2SO3–)-(1→3)-α-l-Fucp; α-l-Fucp-(2,4SO3–)-(1→3)-α-l-Fucp; α-l-Fucp-(4SO3–)-(1→4)-α-l-Fucp; and α-l-Fucp-(4SO3–)-(1→4)-α-l-Fucp-(2SO3–). Both ScF and LlF fucoidans inhibited colony formation and growth of melanoma and colon cancer cells and sensitize-tested cancer cells to X-ray radiation to a comparable degree.