Star-shaped poly(2-aminoethyl methacrylate)s as non-viral gene carriers: Exploring structure-function relationship

[Display omitted] •High-molecular-weight star polymer exhibited enhanced transfection efficiency.•Performance of star polymer was superior to the linear polymer.•Star polymer transfected better in cancer cells than fibroblast-like cells. Gene therapy shows much promise in treating many inheritable a...

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Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2019-09, Vol.181, p.721-727
Main Authors: Chen, Wenjuan, Hong, Yanhang, Zhang, Tao, Kong, Deling, Zhang, Mingming, Zhang, Qiqing, Wang, Chun
Format: Article
Language:English
Subjects:
Animals
Cell Survival - drug effects
Chlorocebus aethiops
COS Cells
Dose-Response Relationship, Drug
Gene transfection
Gene Transfer Techniques
Humans
MCF-7 Cells
Methacrylates - chemical synthesis
Methacrylates - chemistry
Methacrylates - pharmacology
Molecular Structure
Particle Size
Poly(2-aminoethyl methacrylate) (PAEM)
Polymers - chemical synthesis
Polymers - chemistry
Polymers - pharmacology
Star-shaped polycation
Structure-Activity Relationship
Structure-function relationship
Surface Properties
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Summary:[Display omitted] •High-molecular-weight star polymer exhibited enhanced transfection efficiency.•Performance of star polymer was superior to the linear polymer.•Star polymer transfected better in cancer cells than fibroblast-like cells. Gene therapy shows much promise in treating many inheritable and acquired diseases, but challenges remain in the design of gene vectors with low cytotoxicity and high transfection efficiency. Elucidating the structure-function relationship of non-viral polymer-based gene carriers is crucial for improving the design and performance of safe and effective gene therapy approaches. The cationic poly(2-aminoethyl methacrylate) (PAEM) containing primary amino side groups is an attractive carrier for gene delivery. This study focuses on four PAEM-based polycations with well-defined molecular weight and chain architecture. The polymers include three cyclodextrin (CD)-cored star-shaped PAEM polycations (s-PAEM), synthesized by atom transfer radical polymerization (ATRP), and a linear PAEM polycation (l-PAEM), synthesized via activators regenerated by electron transfer (ARGET) ATRP. All four polycations could condense plasmid DNA (pDNA) into spherical polyplexes with small sizes (
ISSN:0927-7765
1873-4367
DOI:10.1016/j.colsurfb.2019.06.029