Enrichment of extracellular vesicles with lipid nanoprobe functionalized nanostructured silica

Nanoscale extracellular vesicles (nEVs) have recently demonstrated potential value in cancer diagnostics and treatment monitoring, but translation has been limited by technical challenges in nEV isolation. Thus, we have developed a one-step nEV isolation platform that utilizes nEV size-matched silic...

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Bibliographic Details
Published in:Lab on a chip 2019-07, Vol.19 (14), p.2346-2355
Main Authors: Wan, Yuan, Maurer, Mackenzie, He, Hong-Zhang, Xia, Yi-Qiu, Hao, Si-Jie, Zhang, Wen-Long, Yee, Nelson S, Zheng, Si-Yang
Format: Article
Language:English
Subjects:
Cancer
Cell Line, Tumor
Extracellular vesicles
Extracellular Vesicles - chemistry
Extracellular Vesicles - pathology
Feasibility Studies
Humans
Lab-On-A-Chip Devices
Lipids
Lipids - chemistry
Microfluidics
Mutation
Nanostructures - chemistry
Pancreatic cancer
Pancreatic Neoplasms - blood
Pancreatic Neoplasms - genetics
Pancreatic Neoplasms - pathology
Proto-Oncogene Proteins p21(ras) - genetics
Silicon dioxide
Silicon Dioxide - chemistry
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Summary:Nanoscale extracellular vesicles (nEVs) have recently demonstrated potential value in cancer diagnostics and treatment monitoring, but translation has been limited by technical challenges in nEV isolation. Thus, we have developed a one-step nEV isolation platform that utilizes nEV size-matched silica nanostructures and a surface-conjugated lipid nanoprobe with an integrated microfluidic mixer. The reported platform has 28.8% capture efficiency from pancreatic cancer plasma and can sufficiently enrich nEVs for simpler positive identification of point mutations, particularly KRAS , in nEV DNA from the plasma of pancreatic cancer patients. Nanoscale extracellular vesicles (nEVs) have recently demonstrated potential value in cancer diagnostics and treatment monitoring, but translation has been limited by technical challenges in nEV isolation.
ISSN:1473-0197
1473-0189
1473-0189
DOI:10.1039/c8lc01359d