Genetic markers enable the verification and manipulation of the dauer entry decision

• Published in: Developmental biology 2019-10, Vol.454 (2), p.170-180
• Main Authors: Shih, Pei-Yin, Lee, James Siho, Sternberg, Paul W.
• Format: Article
• Language: English
• Subjects: Adaptation, Physiological - genetics; Animals; Caenorhabditis elegans - genetics; Caenorhabditis elegans - metabolism; Caenorhabditis elegans Proteins - genetics; Dauer larvae; Diapause; Diapause - genetics; Diapause - physiology; Gene Expression Regulation, Developmental - genetics; Genetic marker; Genetic Markers; Larva - metabolism; Mutation; Neurons - metabolism; Phenotype; Phenotypic plasticity; Signal Transduction
• ISSN: 0012-1606; 1095-564X
• DOI: 10.1016/j.ydbio.2019.06.009

Phenotypic plasticity allows animals to survive in changing environments through the alteration of phenotypes or development. One of the best-studied examples of phenotypic plasticity is dauer larval development in the free-living roundworm Caenorhabditis elegans. When faced with hostile environments, C. elegans larvae can exit reproductive development and enter the stress-resistant and spore-like dauer larval stage. However, knowledge about how the dauer entry decision is made, and how the different tissues of the animal coordinate to execute transformation into dauer, is limited. This is because identifying animals that make the entry decision, or that fail to coordinately remodel their tissues during dauer development, is time-consuming and labor-intensive. Utilizing our previously reported RNA-seq of animals going through dauer or reproductive development (Lee et al., 2017), we have identified genetic markers for conveniently tracking and manipulating the dauer entry decision. These include col-183 (which tracks dauer fate in the hypodermis), ets-10 (neurons and intestine), nhr-246 (intestine and hypodermis), and F53F1.4 (reproductive fate in the hypodermis). Using condition shift experiments, we demonstrate that the dauer-specific fluorescent expression of the markers correspond to the commitment event of the dauer entry decision, and therefore label when the decision is made. We show that these markers can be used to manipulate the entry decision by driving the reproduction-promoting gene daf-9 under the control of the dauer-specific marker col-183, through which we could shift animals into non-dauer development. We further demonstrate that the markers can be used to track tissue coordination during the decision. daf-9, daf-15, and daf-18 partial dauers exhibit incomplete expression of the ets-10 marker, with our results indicating that the same gene (e.g. daf-9 or daf-18) can affect dauer development differently in different tissues. Our findings provide molecular tools for studying phenotypic plasticity during a whole animal decision. •Four genetic markers track the dauer entry decision in distinct tissues.•Fluorescence expression marks the dauer-commitment event in L2d.•The promoters of the markers can be used to manipulate the dauer decision.•Marker expression tracks partial dauer phenotypes and breaks in tissue coordination.