Comparative transcriptome analysis of rhizome nodes and internodes in Panax. japonicus var. major reveals candidate genes involved in the biosynthesis of triterpenoid saponins

The rhizome of P. japonicus var. major, one of the important herbs in Traditional Chinese medicine (TCM), has been used as tonic and hemostatic drugs in Tujia and Miao ethnic groups of China for thousand years. In this study, comparative metabolite and transcriptome analysis of rhizome nodes and int...

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Published in:Genomics (San Diego, Calif.) Calif.), 2020-03, Vol.112 (2), p.1112-1119
Main Authors: Zhang, Shaopeng, Wang, Guang, Zuo, Tian, Zhang, Xiaohai, Xu, Ran, Zhu, Wenjun, You, Jingmao, Wang, Rufeng, Chen, Ping
Format: Article
Language:English
Subjects:
Ginsenosides
Ginsenosides - biosynthesis
Ginsenosides - genetics
Glycosyltransferases - genetics
Glycosyltransferases - metabolism
Metabolome
Panax - genetics
Panax - metabolism
Panax japonicus
Plant Proteins - genetics
Plant Proteins - metabolism
Rhizome
Rhizome - genetics
Rhizome - metabolism
Root Nodules, Plant - genetics
Root Nodules, Plant - metabolism
Transcriptome
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Summary:The rhizome of P. japonicus var. major, one of the important herbs in Traditional Chinese medicine (TCM), has been used as tonic and hemostatic drugs in Tujia and Miao ethnic groups of China for thousand years. In this study, comparative metabolite and transcriptome analysis of rhizome nodes and internodes of wild P. japonicus var. major was performed to reveal their different roles in the biosynthesis of triterpene saponins. The results showed that the node was the crucial section for the synthesis of ginsenosides in the rhizome. The content of oleanane-type ginsenosides in the node was much higher than those in the internode. Most isoprenoid biosynthesis-related genes were highly expressed in the node. And, candidate UDP-glycosyltransferase (UGT) genes were also found to be differentially expressed between node and internode. Our study will provide a better understanding of the metabolism of ginsenosides in the rhizome of P. japonicus var. major. •The content of oleanane-type ginsenosides in rhizome nodes was much higher than those in internodes.•Most isoprenoid biosynthesis-related genes were significantly expressed in the node.•Most UGT genes were significantly differentially expressed between node and internode.•The node was the crucial section for the synthesis of ginsenosides in the rhizome of wild P. japonicus var. major.
ISSN:0888-7543
1089-8646
DOI:10.1016/j.ygeno.2019.06.025