Infectivity and stability of hepatitis C virus in different perfusion solutions

• Published in: Transplant infectious disease 2019-10, Vol.21 (5), p.e13135-n/a
• Main Authors: Helfritz, Fabian A., Wanders, Verena, Bojkova, Denisa, Kuklinski, Nina, Westhaus, Sandra, Swoboda, Sandra, Minor, Thomas, Meuleman, Philip, Paul, Andreas, Steinmann, Eike, Ciesek, Sandra
• Format: Article
• Language: English
• Subjects: Cell culture; Cell Line; Cytotoxicity; HCV infectivity; HCV stability; Hepacivirus - drug effects; Hepacivirus - physiology; Hepatitis; Hepatitis C; Humans; In vitro methods and tests; Infectivity; Organ Preservation Solutions - pharmacology; organ transplantation; Organs; Perfusion; perfusion solutions; Preservation; Replication; Ribonucleic acid; RNA; Stability; Transplantation; Transplants & implants; Virus Attachment - drug effects; Virus Internalization - drug effects; Virus Replication - drug effects; Viruses
• ISSN: 1398-2273; 1399-3062
• DOI: 10.1111/tid.13135

Background Owing to organ shortage, transplantation of organs from HCV (hepatitis C virus) viremic donors into HCV negative individuals is getting more and more accepted. However, transmission of HCV to the host is nearly universal. Until now it is unknown if preservation solutions (PS) might alter infectivity and stability of HCV in the transplant setting. Therefore, seven different preservation solutions (PS) with variable composition were tested in vitro for their direct anti‐ and proviral effects on HCV. Methods In vitro grown HCV based on the JFH‐1 isolate was used to characterize the effect of seven different PS on the HCV replication cycle including HCV attachment, entry, replication, and assembly. In addition, HCV stability in PS was tested. Results Overall, 6/7 PS enhanced HCV infectivity: IGL‐1 increased HCV attachment and entry, UW Belzer and Perfadex boosted HCV entry. Production of novel viral particles was enhanced in HTK, UW Belzer, and IGL‐1. In contrast, viral replication was significantly reduced in HTK solution while all other PS had no effect on HCV RNA replication. HCV was significantly more stable in HTK solution. Euro Collins was the only PS that did not support HCV infectivity in cell culture. None of the used PS showed cytotoxic effects. Conclusion Our data indicate that HCV infectivity and stability is maintained by several PS.