Trypanosoma cruzi extracellular amastigotes selectively trigger the PI3K/Akt and Erk pathways during HeLa cell invasion

Cell invasion by Trypanosoma cruzi extracellular amastigotes involves different signaling pathways to induce phagocytosis-like mechanisms. Previous works indicated that PI3K/Akt, Src and Erk might be involved in EA invasion; however, participation of these molecules in this process remains elusive....

Full description

Saved in:
Bibliographic Details
Published in:Microbes and infection 2019-12, Vol.21 (10), p.485-489
Main Authors: Ferreira, Bianca Lima, Ferreira, Éden Ramalho, Bonfim-Melo, Alexis, Mortara, Renato Arruda, Bahia, Diana
Format: Article
Language:English
Subjects:
Chagas Disease - metabolism
Chagas Disease - parasitology
Enzyme Inhibitors - pharmacology
Extracellular amastigotes
Extracellular Signal-Regulated MAP Kinases - antagonists & inhibitors
Extracellular Signal-Regulated MAP Kinases - metabolism
HeLa Cells
Humans
Phosphoinositide-3 Kinase Inhibitors - pharmacology
Phosphorylation - drug effects
PI3K/Akt - Erk
Proto-Oncogene Proteins c-akt - antagonists & inhibitors
Proto-Oncogene Proteins c-akt - metabolism
Signal Transduction - drug effects
Signaling pathways
Trypanosoma cruzi
Trypanosoma cruzi - physiology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Cell invasion by Trypanosoma cruzi extracellular amastigotes involves different signaling pathways to induce phagocytosis-like mechanisms. Previous works indicated that PI3K/Akt, Src and Erk might be involved in EA invasion; however, participation of these molecules in this process remains elusive. Here, we observed that EA activated Akt, Erk but not Src. Interference of EA invasion with specific inhibitors corroborated this observation. Our results show that EA is capable of selectively triggering complex signaling pathways. Activation of PI3K/Akt and Erk, kinases related to actin cytoskeleton rearrangement and phagocytosis, reinforces the idea that T. cruzi EA subverts the phagocytic machinery during invasion.
ISSN:1286-4579
1769-714X
DOI:10.1016/j.micinf.2019.06.003