High‐salt diet promotes crystal deposition through hypertension in Dahl salt‐sensitive rat model

Objectives To study the promotive effect of salt‐induced hypertension on crystal deposition and urolithiasis using a salt‐sensitive rat hypertension model. Methods Hyperoxaluria and hypercalciuria were induced in male Dahl salt‐sensitive rats with administration of ethylene glycol and alfacalcidol....

Full description

Saved in:
Bibliographic Details
Published in:International journal of urology 2019-08, Vol.26 (8), p.839-846
Main Authors: Nakazawa, Yusuke, Inoue, Shinya, Nakamura, Yuka, Iida, Yasuo, Ishigaki, Yasuhito, Miyazawa, Katsuhito
Format: Article
Language:English
Subjects:
Aldosterone
Angiotensin
Calcium
Dahl salt‐sensitive rats
Diet
DNA microarrays
eplerenone
Ethylene
Ethylene glycol
Gene expression
gene regulation
Hypercalciuria
Hyperoxaluria
Hypertension
Lithiasis
Polymerase chain reaction
Renal function
Renin
RNA-directed DNA polymerase
Salt
salt‐sensitive hypertension
Sodium
Urine
urolithiasis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objectives To study the promotive effect of salt‐induced hypertension on crystal deposition and urolithiasis using a salt‐sensitive rat hypertension model. Methods Hyperoxaluria and hypercalciuria were induced in male Dahl salt‐sensitive rats with administration of ethylene glycol and alfacalcidol. Hypertension was induced by a high‐salt diet. Eplerenone, a selective mineralocorticoid receptor antagonist, was given. Blood and urine were collected to evaluate renal function, electrolytes and the blood renin–angiotensin–aldosterone system. Renal calcium content was also evaluated. Histological examination, transcriptome analysis with DNA microarray and semiquantitative reverse transcriptase polymerase chain reaction were carried out. Results A high‐salt diet increased crystal deposition in Dahl salt‐sensitive rats with hypertension, and eplerenone administration significantly suppressed it. The mRNA expression profile was associated with crystal formation, growth, adhesion and cellular injury, and it was regulated in the group exposed to a high‐salt diet and ethylene glycol. Conclusions A high‐salt diet has a promotive effect on salt‐sensitive hypertension and urolithiasis. This promotive effect can be prevented by eplerenone administration. Hence, salt‐sensitive hypertension has promotive effects on crystal deposition in Dahl salt‐sensitive rats.
ISSN:0919-8172
1442-2042
DOI:10.1111/iju.14035