CRD1, an Xpo1 domain protein, regulates miRNA accumulation and crown root development in rice

Summary Crown root (CR) is the main component of the fibrous root system in cereal crops, but the molecular mechanism underlying CR development is still unclear. Here, we isolated the crown root defect 1 (crd1) mutant from ethyl methane sulfonate‐mutated mutant library, which significantly inhibited...

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Bibliographic Details
Published in:The Plant journal : for cell and molecular biology 2019-10, Vol.100 (2), p.328-342
Main Authors: Zhu, Jianshu, Li, Yong, Lin, Jian, Wu, Yunrong, Guo, Huaxing, Shao, Yanlin, Wang, Fei, Wang, Xiaofei, Mo, Xiaorong, Zheng, Shaojian, Yu, Hao, Mao, Chuanzao
Format: Article
Language:English
Subjects:
adventitious root
Cereal crops
Complementation
Cytoplasm
Disruption
exportin
fibrous root system
Genomes
Levels
microRNA
Mimicry
miRNA
Molecular modelling
Oryza sativa
Phenotypes
Plant cells
Proteins
Rice
root architecture
Root development
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Summary:Summary Crown root (CR) is the main component of the fibrous root system in cereal crops, but the molecular mechanism underlying CR development is still unclear. Here, we isolated the crown root defect 1 (crd1) mutant from ethyl methane sulfonate‐mutated mutant library, which significantly inhibited CR development. The CRD1 was identified through genome resequencing and complementation analysis, which encodes an Xpo1 domain protein: the rice ortholog of Arabidopsis HASTY (HST) and human exportin‐5 (XPO5). CRD1 is ubiquitously expressed, with the highest expression levels in the CR primordium at the stem base. CRD1 is a nucleocytoplasmic protein. The crd1 mutant contains significantly reduced miRNA levels in the cytoplasm and nucleus, suggesting that CRD1 is essential for maintaining normal miRNA levels in plant cells. The altered CR phenotype of crd1 was simulated by target mimicry of miR156, suggesting that this defect is due to the disruption of miR156 regulatory pathways. Our analysis of CRD1, the HST ortholog identified in monocots, expands our understanding of the molecular mechanisms underlying miRNA level and CR development. Significance Statement In this manuscript, our results suggest that CRD1 is essential for maintaining normal levels of miRNA and is required for crown root (CR) development through miR156 in rice. Our findings on CRD1, the HST ortholog identified in monocots, not only provide significant insights into the molecular mechanisms underlying miRNA stability in plant cells, but also establish a hitherto unknown link between regulation of miRNA stability and CR development.
ISSN:0960-7412
1365-313X
DOI:10.1111/tpj.14445