Design of an ultrasound chamber for cellular excitation and observation

In this work, a design of integrating ultrasonic transduction with live cell imaging chamber is introduced. The principle of a metal-incident-glass-output acoustic path was used to deliver a uniform energy profile into the imaging/incubation chamber in the form of leaky Lamb waves. The design was ap...

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Bibliographic Details
Published in:The Journal of the Acoustical Society of America 2019-06, Vol.145 (6), p.EL547-EL553
Main Authors: Chu, Ya-Cherng, Lim, Jormay, Hong, Cheng-Wei, Chu, Yeh-Shiu, Wang, Jaw-Lin
Format: Article
Language:English
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Summary:In this work, a design of integrating ultrasonic transduction with live cell imaging chamber is introduced. The principle of a metal-incident-glass-output acoustic path was used to deliver a uniform energy profile into the imaging/incubation chamber in the form of leaky Lamb waves. The design was applied to examine living mouse mammary gland epithelial cells (EpH4). Significant changes in intracellular activities were observed even at a very low energy intensity level (1 MHz, ISATA = 1 mW/cm2, continuous wave). Live imaging with ultrasonic stimulation provides a different paradigm to interrogate cellular mechanosensitive responses in real time.
ISSN:0001-4966
1520-8524
DOI:10.1121/1.5111974