Lychnophora pinaster ethanolic extract and its chemical constituents ameliorate hyperuricemia and related inflammation

• Published in: Journal of ethnopharmacology 2019-10, Vol.242, p.112040-112040, Article 112040
• Main Authors: Martins de Sá Müller, Camila, Coelho, Grazielle Brandão, Carolina de Paula Michel Araújo, Marcela, Saúde-Guimarães, Dênia Antunes
• Format: Article
• Language: English
• Subjects: Animals; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Asteraceae; Brazilian arnica; E-lychnophoric acid; Ethanol - chemistry; Gout; Gout Suppressants - chemistry; Gout Suppressants - pharmacology; Gout Suppressants - therapeutic use; Hexanes - chemistry; Hyperuricemia; Hyperuricemia - blood; Hyperuricemia - drug therapy; Inflammation; Liver - drug effects; Liver - enzymology; Male; Mice; Phytochemicals - analysis; Phytochemicals - pharmacology; Phytochemicals - therapeutic use; Plant Components, Aerial; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; Solvents - chemistry; Uric Acid - blood; Xanthine oxidase; Xanthine Oxidase - antagonists & inhibitors; Xanthine Oxidase - metabolism
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2019.112040

Lychnophora pinaster, known as “Brazilian arnica” is used in folk medicine as alcoholic extract to treat inflammation, pain, rheumatism and bruises. Evaluate the effects of the Lychnophora pinaster's ethanolic extract and its chemical constituents on inflammation and hyperuricemia. Ethanolic and hexanic extracts were obtained from the aerial parts of L. pinaster. Sesquiterpene E-lychnophoric acid was isolated from hexanic extract and identified by RMN, GC/MS and IR. In vivo anti-hyperuricemic and anti-inflammatory effects of the ethanolic extracts from L. pinaster (40, 125, 375 mg/kg), E-lychnophoric acid and other constituents previous isolated from L. pinaster and identified in the ethanolic extract by HPLC/UV/DAD (rutin, quercetin and vitexina flavonoids, caffeic, cinnamic and chlorogenic acids, lupeol and stigmasterol, at dose of 15 mg/kg) were assayed by experimental model of oxonate-induced hyperuricemia in Swiss mice, liver xanthine oxidase (XOD) inhibition and by MSU-induced paw edema in mice. Ethanolic extract and all its components presented anti-hyperuricemic activity by inhibiting the hepatic xanthine oxidase activity. Ethanolic extract and its chemical constituents, except quercetin and vitexin, were able to reduce paw edema size induced by urate crystals. Hypouricemic and anti-inflammatory results obtained for the ethanolic extract (40, 125, 375 mg/kg) and E-lychnophoric acid (15 mg/kg) were similar those obtained for standard drugs, allopurinol (10 mg/kg) and indomethacin (3 mg/kg). Ethanolic extract and E-lychnophoric, chlorogenic, cinnamic and caffeic acids, rutin, lupeol and stigmasterol presented anti-inflammatory and anti-hyperuricemic actvities. These compounds are responsible for the activities presented by the ethanolic extract of L. pinaster. Ethanolic extract and its chemical constituents can be considered promising agents in the therapeutic of inflammation, hyperuricemia and gout. [Display omitted]