Replication fork pausing at protein barriers on chromosomes

When a cell divides prior to completion of DNA replication, serious DNA damage may occur. Thus, in addition to accuracy, the processivity of the replication forks is important. DNA synthesis at replication forks should be completed in time, and forks overcome aberrant structures on the template DNA,...

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Bibliographic Details
Published in:FEBS letters 2019-07, Vol.593 (13), p.1449-1458
Main Authors: Hizume, Kohji, Araki, Hiroyuki
Format: Article
Language:English
Subjects:
Base Sequence
Chromosomes - genetics
CMG complex
DNA polymerase
DNA Replication
DNA-Binding Proteins - metabolism
Fob1
fork pausing
fork protection complex
helicase
Polε
programmed fork arrest
replication fork
replication fork barrier
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Summary:When a cell divides prior to completion of DNA replication, serious DNA damage may occur. Thus, in addition to accuracy, the processivity of the replication forks is important. DNA synthesis at replication forks should be completed in time, and forks overcome aberrant structures on the template DNA, including damaged sites, using trans‐lesion synthesis, occasionally introducing mutations. By contrast, the protein barrier built on the DNA is known to block the progression of replication forks at specific chromosomal loci. Such protein barriers avert any collision of replication and transcription machineries, or control the recombination of specific loci. The components and the mechanisms of action of protein barriers have been revealed mainly using genetic and biochemical techniques. In addition to proteins involved in replication fork pausing, the interaction of the replicative helicase and DNA polymerase is also essential for replication fork pausing. Here, we provide an overview of replication fork pausing at protein barriers.
ISSN:0014-5793
1873-3468
DOI:10.1002/1873-3468.13481