Associations Between Loss of ARID1A Expression and Clinicopathologic and Genetic Variables in T1 Early Colorectal Cancer

• Published in: American journal of clinical pathology 2019-09, Vol.152 (4), p.463-470
• Main Authors: Kishida, Yoshihiro, Oishi, Takuma, Sugino, Takashi, Shiomi, Akio, Urakami, Kenichi, Kusuhara, Masatoshi, Yamaguchi, Ken, Kitagawa, Yuko, Ono, Hiroyuki
• Format: Article
• Language: English
• Subjects: Adenine; Adenocarcinoma - genetics; Adenocarcinoma - metabolism; Adenocarcinoma - pathology; Adult; Age Factors; Aged; Aged, 80 and over; Cancer; Care and treatment; Colorectal cancer; Colorectal carcinoma; Colorectal Neoplasms - genetics; Colorectal Neoplasms - metabolism; Colorectal Neoplasms - pathology; Development and progression; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Drosophila Proteins; Female; Gene expression; Genetic aspects; Genetic variation; Health aspects; High-Throughput Nucleotide Sequencing; Humans; Immunohistochemistry; Lymph nodes; Lymphatic Metastasis - genetics; Lymphatic Metastasis - pathology; Male; Membrane Proteins; Metastases; Middle Aged; MSH6 protein; Mutation; Next-generation sequencing; Proto-Oncogene Proteins p21(ras) - genetics; Proto-Oncogene Proteins p21(ras) - metabolism; Retrospective Studies; Thymine; Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Tumors
• ISSN: 0002-9173; 1943-7722
• DOI: 10.1093/ajcp/aqz062

Abstract Objectives To evaluate the relationships between adenine-thymine-rich interactive domain 1A (ARID1A) expression and the clinicopathologic features in T1 colorectal cancer (CRC) and to investigate whether the presence of ARID1A protein is related to genetic changes. Methods We retrospectively studied 219 surgically resected T1 CRCs. ARID1A expression was assessed by immunohistochemical methods, and the correlation between ARID1A expression and clinicopathologic features was evaluated. The relationship between ARID1A expression and 409 cancer-related gene mutations was also evaluated using next-generation sequencing (NGS). Results Immunohistochemical staining indicated negative ARID1A expression in 4.6%. Loss of ARID1A expression was significantly associated with younger age, lymphatic invasion, and lymph node metastasis (LNM). NGS showed that PKHD1, RNF213, and MSH6 mutations were more frequent in ARID1A-negative tumors, whereas KRAS mutations were more common in ARID1A-positive tumors. Conclusions In T1 CRC, negative ARID1A expression was correlated with early onset, lymphatic invasion, and LNM. Mutations in some cancer-related genes were possibly related with ARID1A expression.