Identification of Novel Butyrate- and Acetate-Oxidizing Bacteria in Butyrate-Fed Mesophilic Anaerobic Chemostats by DNA-Based Stable Isotope Probing

Butyrate is one of the most important intermediates during anaerobic digestion of protein wastewater, and its oxidization is considered as a rate-limiting step during methane production. However, information on syntrophic butyrate-oxidizing bacteria (SBOB) is limited due to the difficulty in isolati...

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Bibliographic Details
Published in:Microbial ecology 2020-02, Vol.79 (2), p.285-298
Main Authors: Yi, Yue, Wang, HuiZhong, Chen, YaTing, Gou, Min, Xia, ZiYuan, Hu, Bin, Nie, Yong, Tang, YueQin
Format: Article
Language:English
Subjects:
Acetates
Acetic acid
Anaerobic digestion
Anaerobic treatment
Bacteria
Biodegradation
Biomedical and Life Sciences
Carbon sources
Chemostats
Deoxyribonucleic acid
Dilution
DNA
DNA sequencing
Ecology
ENVIRONMENTAL MICROBIOLOGY
Geoecology/Natural Processes
Intermediates
Life Sciences
Metabolism
Microbial Ecology
Microbiology
Nature Conservation
Next-generation sequencing
Oxidation
rRNA 16S
Species
Spirochaetaceae
Stable isotopes
Synergistaceae
Wastewater
Water Quality/Water Pollution
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Summary:Butyrate is one of the most important intermediates during anaerobic digestion of protein wastewater, and its oxidization is considered as a rate-limiting step during methane production. However, information on syntrophic butyrate-oxidizing bacteria (SBOB) is limited due to the difficulty in isolation of pure cultures. In this study, two anaerobic chemostats fed with butyrate as the sole carbon source were operated at different dilution rates (0.01/day and 0.05/day). Butyrate- and acetate-oxidizing bacteria in both chemostats were investigated, combining DNA-Stable Isotope Probing (DNA-SIP) and 16S rRNA gene high-throughput sequencing. The results showed that, in addition to known SBOB, Syntrophomonas, other species of unclassified Syntrophomonadaceae were putative butyrate-oxidizing bacteria. Species of Mesotoga, Aminivibrio, Acetivibrio, Desulfovibrio, Petrimonas, Sedimentibacter, unclassified Anaerolineae, unclassified Synergistaceae, unclassified Spirochaetaceae, and unclassified bacteria may contribute to acetate oxidation from butyrate metabolism. Among them, the ability of butyrate oxidation was unclear for species of Sedimentibacter, unclassified Synergistaceae, unclassified Spirochaetaceae, and unclassified bacteria. These results suggested that more unknown species participated in the degradation of butyrate. However, the corresponding function and pathway for butyrate or acetate oxidization of these labeled species need to be further investigated.
ISSN:0095-3628
1432-184X
DOI:10.1007/s00248-019-01400-z