Differential Response of BEAS-2B and H-441 Cells to Methylene Blue Photoactivation

Cancer incidence and mortalities are growing worldwide, therefore research and development of more effective and less invasive treatments, such as photodynamic therapy, are needed. Herein, we investigated the methylene blue (MB) photoactivation effects in lung epithelial cells (BEAS-2B) and lung ade...

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Bibliographic Details
Published in:Anticancer research 2019-07, Vol.39 (7), p.3739-3744
Main Authors: Rodríguez-Córdova, Rosalva Josefina, Gutiérrez-Valenzuela, Cindy Alejandra, Bojang, Pasano, Esquivel, Reynaldo, Hernández, Pedro, Ramos, Kenneth S, Guzmán-Zamudio, Roberto, Lucero-Acuña, Armando
Format: Article
Language:English
Subjects:
Adenocarcinoma
Adenocarcinoma - drug therapy
Adenocarcinoma - metabolism
Aqueous solutions
Cancer
Cell Line
Cell Survival - drug effects
Cell viability
Colorimetry
Epithelial cells
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Humans
Invasiveness
Light
Lung cancer
Lung Neoplasms - drug therapy
Lung Neoplasms - metabolism
Lungs
Methylene blue
Methylene Blue - pharmacology
Photoactivation
Photochemotherapy
Photodynamic therapy
Photosensitizing Agents - pharmacology
R&D
Reactive oxygen species
Reactive Oxygen Species - metabolism
Research & development
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Summary:Cancer incidence and mortalities are growing worldwide, therefore research and development of more effective and less invasive treatments, such as photodynamic therapy, are needed. Herein, we investigated the methylene blue (MB) photoactivation effects in lung epithelial cells (BEAS-2B) and lung adenocarcinoma cells (H-441). The reactive oxygen species (ROS) produced by the laser photoactivation of MB in aqueous solutions and cell cultures were measured with probes, and the cell viability was evaluated with a colorimetric assay. MB up to 31.26 μM did not induce detectable effects in BEAS-2B cells. However, H-441 cells presented adverse effects below that concentration in the same range of fluencies studied. These results are in concordance with the ROS production in H-441 cells, while in BEAS-2B cells the production of ROS was less significant compared to the control. Photoactivation of MB at concentrations below 31.26 μM could be used for the selective treatment of H-441 cells over non-cancer cells.
ISSN:0250-7005
1791-7530
DOI:10.21873/anticanres.13522